Glucobrassicin-Brussel Sprout Effect on D10 Phe Metabolism

University of Minnesota (UMN)

Status and phase

Completed

Phase 1

Conditions

Smoking

Treatments

Drug: Deuterated Phenanthrene

Other: Brussel Sprouts

Study type

Interventional

Funder types

Other

Identifiers

NCT02999399

1610M96281

Details and patient eligibility

About

To determine whether glucobrassicin-rich Brussels sprouts can favorably modify the metabolism of the polycyclic aromatic hydrocarbon deuterated phenanthrene ([D10]phe) in current and former smokers

Full description

Subjects will be given 1 microgram of [D10]phe, and all urine will be collected for 6 h afterwards to quantify baseline levels of [D10]phenanthrene tetraol ([D10]pheT) and [D10]phenanthrols ([D10]HOP). Within 3 days of this baseline measurement, subjects will consume 200 micromoles of glucobrassicin in the form of raw Brussels sprouts (~200-300 grams) at the study center once daily for 7 consecutive days. Urine will be collected for 24 h after vegetable consumption on days 3 ± 1 and 6 of the feeding intervention for 3,3'-diindolylmethane (DIM) quantification. On day 7 of the feeding intervention, a second dose of 1 microgram of [D10]phe will be administered at the study center after vegetable consumption, followed by another 6 h urine collection.

Enrollment

39 patients

Sex

All

Ages

18 to 99 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Adult ≥ 18 years old
Current or former smoker by self-report
Willing to abstain from cruciferous vegetable consumption other than the study vegetable during the study period
Able to understand the experimental nature of the study and provide informed consent
Negative urine pregnancy test for women of childbearing potential within 7 days of baseline [D10]phe dosing

Exclusion criteria

Chronic proton pump inhibitor, H2-blocker (i.e., ranitidine, famotidine), and/or calcium carbonate use
History of gastric bypass surgery, gastric banding, bowel resection, malabsorption syndromes such as celiac sprue or pancreatic insufficiency, or other conditions that may affect gastric absorption
Current use of tobacco products other than cigarettes (i.e. snuff, snuz, smokeless tobacco, cigars, pipes) or use of these products within 3 months of study registration
Major or chronic medical disease, including heart disease, poorly controlled diabetes, etc., to be adjudicated by the principal investigator
Women who are pregnant, intend to become pregnant within 3 months of the study period, or who are breastfeeding
Current or recent (within 12 months) problems with drug use or alcohol dependence by self-report Antibiotic use within 2 months of study enrollment or during the study by self-report Alcohol dependence, abuse, or history of dependence/abuse by self-report Vegetarians
History of respiratory tract cancer
Use of metronidazole or antabuse during the study
Taking ibuprofen, naproxen, other NSAIDs, steroids (except inhaled steroids) within 14 days of study registration
Allergy to Brussels sprouts