Glucotoxicity and Acute Exercise

Rigshospitalet

Status: Withdrawn

Conditions: Type 1 Diabetes; Type 2 Diabetes

Treatments: Procedure: Hyperglycemia; Behavioral: Exercise

Study type: Interventional

Funder types: Other

Identifiers

NCT01771614

GlucotoxEx

Details and patient eligibility

About

Experimental hyperglycemia (5 hours) will be induced via a constant- or fluctuating-rate glucose infusion to establish different patterns of glycemia in healthy individuals. The effects of these acute models of hyperglycemia on the adaptations to a single aerobic exercise bout will be tested. Changes in pancreatic endocrine function, insulin sensitivity, endothelial function, and oxidative stress will be measured.

Sex: Male

Ages: 18 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion criteria for participation:

• Male
• Age 18-50 years
• Body Mass Index 19-30 kg/m2
• Generally healthy

Exclusion criteria for participation:

• Regular use of anti-inflammatory medication
• Smokers
• More than 2 kg weight change in prior 6 months
• Previous weight loss surgery
• Cancer, or evidence of chronic haematological, pulmonary, cardiac, hepatic, renal, or gastrointestinal disease
• Evidence of diabetes
• Engaged in moderate or vigorous activity on more than 5 days per week

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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0 participants in 3 patient groups

Normoglycemia + exercise

Experimental group

Description:

At T = 0 h participants will undergo an intravenous glucose tolerance test (IVGTT, 0.33 g/kg glucose) immediately followed by a 4-hour rest period. At T = 5 h, participants will undertake 45 minutes of moderate-intensity (70% VO2max) bicycle ergometry. Immediately following (T = 6 h) and 1- (T = 7 h), 3- (T = 9 h), and 18- hours (T = 24 h) after exercise, participants will undergo additional IVGTTs.

Treatment:

Behavioral: Exercise

Steady-State Hyperglycemia + exercise

Experimental group

Description:

At T = 0 h participants will undergo an intravenous glucose tolerance test (IVGTT, 0.33 g/kg glucose) immediately followed by a 4-hour rest period. During this time, steady-state hyperglycemia (~10 mM) will be induced experimentally via a variable-rate intravenous infusion of 20% dextrose. At T = 5 h, participants will undertake 45 minutes of moderate-intensity (70% VO2max) bicycle ergometry. Immediately following (T = 6 h) and 1- (T = 7 h), 3- (T = 9 h), and 18- hours (T = 24 h) after exercise, participants will undergo additional IVGTTs.

Treatment:

Behavioral: Exercise

Procedure: Hyperglycemia

Fluctuating Hyperglycemia + exercise

Experimental group

Description:

At T = 0 h participants will undergo an intravenous glucose tolerance test (IVGTT, 0.33 g/kg glucose) immediately followed by a 4-hour rest period. During this time, fluctuating hyperglycemia (~8-15 mM) will be induced by intravenously injecting 0.15 g/kg boluses of 20% dextrose every 30 minutes. At T = 5 h, participants will undertake 45 minutes of moderate-intensity (70% VO2max) bicycle ergometry. Immediately following (T = 6 h) and 1- (T = 7 h), 3- (T = 9 h), and 18- hours (T = 24 h) after exercise, participants will undergo additional IVGTTs.

Treatment:

Behavioral: Exercise

Procedure: Hyperglycemia