HPV as a Possible Cause of Azoospermia

Odense University Hospital

Status: Completed

Conditions: HPV in Testis Tissue

Treatments: Procedure: Testicular biopsy

Study type: Observational

Funder types: Other

Identifiers

NCT02329275

Lab.Reprod.Biol.- Odense.02

Details and patient eligibility

About

Azoospermia is found in 5-20% of all men suffering from infertility, and in more than 20% azoospermia remains unexplained. The investigators suggest, that HPV may be an etiological factor in azoospermia and reduced sperm production.

The investigators plan to examine the occurrence of HPV in testicular tissue from 100 azoospermic men and to compare with the HPV occurrence in testicular tissue from a control group of 40 normal, healthy men undergoing vasectomy. Since HPV, in a recent Danish study, was detected on sperm in 16% of 188 randomly selected semen donors, the investigators also plan to examine the occurrence of HPV on sperm ejaculated during the last week before vasectomy. If HPV should be found in testicular tissue from men belonging to the control group, it will be highly relevant to compare with the HPV occurrence on sperm ejaculated from the men in concern.

A Linear Array HPV Genotyping Kit from Roche will be used for detection of HPV in testicular tissue and ejaculates. In total, with this test kit 37 HPV genotypes are examined for, including 13 high risk types.

Enrollment: 140 patients

Sex: Male

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Men with azoospermia (Group 1) and proven fertile men undergoing vasectomy (Group 2)

Exclusion criteria

• Men with testicular abnormalities (Group 2)
• Men who do not understand the purpose and possible consequences of the study (both groups)

Trial design

140 participants in 2 patient groups

Azoospermic men

Treatment:

Procedure: Testicular biopsy

Men with proven fertility

Treatment:

Procedure: Testicular biopsy