Human Chorionic Gonadotropin Stimulation Effects on Steroidogenesis in Men With Klinefelter Syndrome

Azienda USL Modena

Status: Completed

Conditions: Klinefelter Syndrome; Steroidogenesis; Human Chorionic Gonadotropin (hCG) Stimulation

Treatments: Drug: human chorionic gonadotropin

Study type: Interventional

Funder types: Other

Identifiers

NCT02788136

Klinefelter & hCG

Details and patient eligibility

About

The study design included six visits. During the first visit (visit 0), the subjects underwent physical examination(height, weight, body mass index (BMI), arm span, and upper segment measurement) and testicular ultrasound (US) for the calculation of testicular volume. At 0800 h of day 0, all subjects provided a basal blood sample immediately followed by a single intramuscular injection of hCG of 5000 IU. Further five visits were performed each of five following consecutive days after the hCG injection. A blood sample was taken at each visit after an overnight fast

Full description

Blood samples were centrifuged at 2955 g for 15 min. Sera were transferred into plain polypropylene tubes and stored at -20°C until assayed. P, 17OHP, AS, DHEA, and TS were determined by LC-MS/MS at the laboratory of the Centre for Applied Biomedical Research of the S. Orsola-Malpighi Hospital, Alma Mater Studiorum, University of Bologna, Bologna, Italy.

Enrollment: 25 patients

Sex: Male

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Klinefelter syndrome diagnosis at kariotype evaluation for the study group
• Healthy age-matched men in the control group

Exclusion criteria

• previous or ongoing androgen therapy

Trial design

25 participants in 2 patient groups

Klinefelter syndrome

Experimental group

Description:

13 men with diagnosis of Klinefelter syndrome were stimulated with human chorionic gonadotropin (hCG)

Treatment:

Drug: human chorionic gonadotropin

Control

Active Comparator group

Description:

12 healthy age-related men were stimulated with human chorionic gonadotropin (hCG)

Treatment:

Drug: human chorionic gonadotropin