Hypoxia and Kynurenine Pathway in Periodontal Inflammation

Medipol Health Group

Status: Completed

Conditions: Periodontitis; Diagnose Disease

Treatments: Other: Saliva and serum sampling

Study type: Observational

Funder types: Other

Identifiers

NCT07182448

1124

Details and patient eligibility

About

The kynurenine pathway (KP), the primary route for tryptophan (TRP) metabolism, is activated during inflammation and hypoxic conditions, contributing to immune dysregulation and oxidative stress. the interconnected roles of hypoxia, KP activation, and oxidative stress in periodontitis have not been comprehensively investigated.The aim of this study was to determine the role of the kynurenine (KYN) pathway and hypoxia in periodontal inflammation, as well as their association with periodontal clinical parameters.

Full description

Systemically healthy 23 stage III, grade B periodontitis patients and 22 periodontally healthy individuals were included in this study. Salivary and serum tumor necrosis factor-alpha (TNF-α), hypoxia-inducible factor-1 alpha (HIF-1α) and 8-hydroxy-2'-deoxyguanosine (8-OhdG) levels were evaluated by ELISA, and salivary and serum KP metabolites were evaluated by liquid chromatography-mass spectrometry.

Enrollment: 45 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• systemically healthy
• clinical diagnosis of periodontitis
• clinical diagnosis of periodontal health

Exclusion criteria

• decline to participate and being under 18 & over 65 years of age;
• having any systemic inflammatory diseases such as diabetes, rheumatoid arthritis, or systemic conditions, including immunodeficiency syndrome, cardiovascular disorders, or hepatic disorders;
• the use of antibiotics and/or anti-inflammatory nonsteroidal anti-inflammatory drugs within 3 months preceding the study;
• having nonsurgical periodontal treatment (previous 6 months);
• having surgical periodontal treatment (previous 12 months);
• having <20 natural teeth excluding the third molars.

Trial design

45 participants in 2 patient groups

Saliva and serum collection of patients and samples molecules analysis

Description:

Saliva and serum collection of patients and samples molecules analysis Saliva and serum sampling Saliva were collected to analyze the selected markers as unstimulated samples during the early hours of the day. The saliva was centrifuged and then transferred into Eppendorf tubes. Venous puncture was performed after saliva collection and 10 mL of blood samples were collected by qualified staff from each participant. Saliva and serum were then stored at -80 °C until analysis.

Treatment:

Other: Saliva and serum sampling

Salivary and serum TNF-α, HIF-1α, 8-OhdG , KYN, TRP observation

Description:

Salivary and serum tumor necrosis factor-alpha (TNF-α), hypoxia-inducible factor-1 alpha (HIF-1α) and 8-hydroxy-2'-deoxyguanosine (8-OhdG) levels were evaluated by ELISA, and salivary and serum KP metabolites were evaluated by liquid chromatography-mass spectrometry.

Treatment:

Other: Saliva and serum sampling