Identification of Anaphylactogenic Antibodies in Peanut Allergy

University of Zurich (UZH)

Status: Invitation-only

Conditions: Peanut Allergy

Treatments: Other: Observational study

Study type: Observational

Funder types: Other

Identifiers

NCT02821286

UZH

Details and patient eligibility

About

This project intends the analysis and profiling of specific antibodies against major peanut allergens in peanut allergic individuals and molecular cloning of human antibodies against major peanut allergens.

Full description

• Characterization of patient samples reactivity (sera obtained from blood) against major peanut allergens.
• Isolation of antibody-producing B cells.
• Molecular cloning of peanut-specific antibodies from antibody gene sequences obtained from B cells.
• Biophysical and functional characterization of isolated antibodies

Enrollment: 100 estimated patients

Sex: All

Ages: 3+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Participants have a described clinical history of peanut allergy and/or are sensitized to peanut allergens (as evidenced by positive skin prick test and allergen-specific IgE testing)
• Male and female subjects older than 3 years
• Written informed consent

Exclusion criteria

• age below three years
• patients suffering from any disease where blood withdrawals might impact the patients' health status such as known anemia.