Identify Bacteria Associated With Metabolic Diseases That Reside Both in Intestinal Crypts and in Blood (MICIMAB)

Toulouse University Hospital

Status: Completed

Conditions: Obesity

Treatments: Other: patients free type 2 diabetes; Other: diabetic patients; Other: obese patients; Other: patients free of obesity and diabetes

Study type: Interventional

Funder types: Other

Identifiers

NCT02261831

13 6936 03

Details and patient eligibility

About

The investigators will compare 16sRDNA profile in cases and controls in order to identify bacteria associated with metabolic diseases that reside both in intestinal crypts and in blood.

Full description

Experimental evidence suggests that bacterial translocation from gut to tissue could be an initial step leading to diabetes. To test this hypothesis, a bacterial component that is highly conserved between different species of bacteria, the 16S rDNA gene will be analysed both in intestinal crypt and in blood in patients with type 2 diabetes, obese patients and control.

Enrollment: 99 patients

Sex: All

Ages: 35 to 75 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Between 35 and 75 years old
• Colonoscopy and biopsies required

Diabetes population:

• Type 2 diabetes

Obese population:

• Android obesity

Exclusion criteria

• Inflammatory bowel disease
• Colonic cancer
• Bariatric surgery
• Previous administration of antibiotic (within 15 days)
• Pregnancy

Trial design

Primary purpose: Diagnostic

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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99 participants in 4 patient groups

obese patients

Experimental group

Description:

Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies

Treatment:

Other: obese patients

Diabetic patients

Experimental group

Description:

Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies

Treatment:

Other: diabetic patients

Patients free of obesity and diabetes

Experimental group

Description:

Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies

Treatment:

Other: patients free of obesity and diabetes

patients free type 2 diabetes.

Experimental group

Description:

Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies

Treatment:

Other: patients free type 2 diabetes