Identifying Cancer Genes in in Blood and Bone Marrow Samples From Patients With Acute Myeloid Leukemia

Alliance for Clinical Trials in Oncology

Status: Completed

Conditions: Leukemia

Treatments: Genetic: polymerase chain reaction; Genetic: microarray analysis

Study type: Observational

Funder types: Other; NIH

Identifiers

NCT00897182

NCI-2009-00462 (Registry Identifier)

CALGB-20801

CDR0000595708 (Registry Identifier)

Details and patient eligibility

About

RATIONALE: Studying samples of blood and bone marrow in the laboratory from patients with cancer may help doctors learn more about changes that occur in DNA and identify genes related to cancer. It may also help doctors diagnose cancer and predict how patients will respond to treatment.

PURPOSE: This research study is identifying cancer-related genes in blood and/or bone marrow samples from patients with acute myeloid leukemia.

Full description

OBJECTIVES:

• To identify and validate individual genes for diagnosis of three major translocations in acute myeloid leukemia.
• To correlate transcript expression data in the various translocations with age, sex, race, response to treatment, and survival and with other known mutations.

OUTLINE: Blood and/or bone marrow samples previously procured from patients on CALGB-9665 are obtained from the CALGB Leukemia Tissue Bank from patients enrolled on CALGB AML treatment studies. Mononuclear cells are isolated from samples and mRNA is extracted. Gene expression profiles are analyzed via custom mRNA microarray and confirmed by quantitative real-time PCR.

Enrollment: 96 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

• Enrolled on CALGB acute myeloid leukemia (AML) treatment studies AND concurrently enrolled on Leukemia Tissue Bank Protocol CALGB-9665

• Short-term or long-term survivor

• Bone marrow and/or peripheral blood obtained at diagnosis

• Leukemia is one of the following cytogenetic subtypes:

  • t(8;21)
  • t(15;17)
  • inv(16)

Trial design

96 participants in 1 patient group

Group 1

Description:

This is a CALGB Leukemia Tissue Bank project makes use of tissue from patients who have previously provided their consent. Diagnostic samples from patients enrolled on CALGB AML treatment studies (eg, CALGB 9621, 9710, and 19808) and who have been registered on the companion Leukemia Tissue Bank Protocol CALGB 9665 were used.

Treatment:

Genetic: microarray analysis

Genetic: polymerase chain reaction