IL-32 Isoforms Levels in GCF and Plasma of the Patients With Periodontitis

Bulent Ecevit University

Status

Completed

Conditions

Periodontitis

Treatments

Other: Non-surgical periodontal treatment

Study type

Observational

Funder types

Other

Identifiers

NCT04350489

AP-1720

Details and patient eligibility

About

Interleukin (IL)-32, which has been recently reported to be associated with periodontitis, has been suggested to have pleiotropic effect due to its 9 different isoforms. The aim of this study was to investigate the levels of IL-32α, IL-32β, IL-32γ, IL-32δ isoforms in gingival crevicular fluid (GCF) and plasma before and after nonsurgical periodontal treatment in patients with periodontitis (P).Twenty-seven P and 27 periodontally healthy controls (C) were recruited in this study. Periodontitis patients were performed nonsurgical periodontal treatment. GCF and plasma sampling and clinical periodontal parameters were evaluated before and 1 month after treatment. Enzyme-linked immunosorbent assay was used to analyze the levels of IL-32α, IL-32β, IL-32γ, IL-32δ isoforms in GCF and plasma samples.

Enrollment

54 patients

Sex

All

Ages

25 to 55 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Have at least 20 natural teeth, excluding third molars.
Periodontitis patients had at least two non-adjacent sites per quadrant with probing depth (PD) ≥ 5 mm and clinical attachment level (CAL) ≥ 5 mm with gingival inflammation, and alveolar bone loss affecting >30% of the teeth, as detected on clinical and radiographical examinations.
Periodontally healthy control group had no sign of gingival inflammation, no PD > 3mm and no evidence of attachment or bone loss

Exclusion criteria

History of systemic disease.
Regular use of any drugs which can effect the immune system or inflammatory response in the 6 months preceding the start of the study.
Periodontal treatment during last 6 months that could affect periodontal status.
Smoking.
History of radiotherapy or chemotherapy.
Current pregnancy, lactation or menopause.