IL-8 Receptor-modified CD70 CAR T Cell Therapy in CD70+ Pediatric High-grade Glioma (HGG) (IMPACT)

University of Florida

Status and phase

Enrolling

Phase 1

Conditions

High-grade Glioma

Treatments

Biological: Ex-Vivo expanded autologous IL-8 receptor (CXCR2) modified CD70 CAR (8R-70CAR) T cells

Study type

Interventional

Funder types

Other

Identifiers

NCT06946680

OCR41673 (Other Identifier)

IRB202200057-P

Details and patient eligibility

About

This is a phase I study to assess the safety and feasibility of IL-8 receptor modified patient-derived activated CD70 CAR T cell therapy in CD70+ pediatric high-grade glioma

Full description

Identified newly-diagnosed pediatric CD70+ HGG patients will be enrolled in this clinical trial study prior to initiation of standard-of-care chemo-radiation. Prior to initiation of chemoradiation, PBMCs will be collected through peripheral venipuncture. After tumor CD70 status is confirmed, the 8R-70CAR T cell production will start.

4 weeks (+/- 1) post completion of radiation, pediatric patients, based on institutional policy, will initiate adjuvant chemo with dose-intensified TMZ 75-100 mg/m2/day x 21 days for up to 3 cycles. 8R-70CAR T cells will be administered at day 21-24 of the TMZ cycle as a single intravenous (IV) infusion, or for pediatric patients not receiving adjuvant chemo once 8R-70CAR T cells. Pediatric patients will receive lymphodepletion prior to CAR T cell administration.

Enrollment

18 estimated patients

Sex

All

Ages

4 to 18 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Newly-diagnosed pHGG based on the absence of a previous history of brain tumor (WHO Grade III-IV glioma) by histopathology.
CD70 positive (≥5%, 1+) The tumors from the surgical resection by immunohistochemistry will be confirmed by a validated assay performed at UF Health Pathology, a certified Lab.

o CD70 tumor expression performed on paraffin-embedded tumor specimens will be evaluated. Tumor expression will be scored on a scale of 0 to 3 staining intensity: 0 = Negative
1. = Low level
2. = Moderate level
3. = High level The criteria for inclusion will be at least 5% of the cells scoring 1+ staining intensity (> 5%, 1+).
Karnofsky Performance Status (KPS) or Lansky Performance Score (LPS) of > 70% (Appendix C) Patients who are unable to walk because of neurologic deficits, but who are up in a wheelchair, will be considered ambulatory for the purpose of assessing the performance score provided the neurological deficit is stable.
CBC with differential with adequate bone marrow function as defined below:
- Absolute neutrophil count (ANC) ≥ 1000 cells/mm3.
- Platelet count ≥ 100,000 cells/mm3.
- Hemoglobin ≥ 10 g/dl. (The use of transfusion or other intervention to achieve Hgb ≥ 10 g/dl is acceptable.)
Adequate renal function as defined below:

o Serum creatinine < 1.5 x institutional upper limit of normal for age and gender. Patients who do not meet the criteria but have a 24-hour Creatinine Clearance or GFR (radioisotope or iothalamate) ≥ 70 mL/min/1.73 m2 are eligible
Adequate hepatic function as defined below:
- Total bilirubin ≤ 1.5 x institutional upper limit of normal (ULN) for age
- ALT ≤ 3 times institutional upper limits of normal for age
- AST ≤ 3 times institutional upper limits of normal for age
Signed informed consent, or for patients age <18, parental permission, and, as appropriate, assent from pediatric patients age ≥12. If the patient's mental status precludes their informed consent, the legally authorized representative may give informed consent. Consent or permission/assent will be obtained at screening (before PBMC collection) and before treatment with CAR T-cells.
For females of childbearing potential, a negative serum pregnancy test at enrollment.
Women of childbearing potential (WOCBP) must be willing to use an acceptable contraceptive method to avoid pregnancy throughout the study and for at least 24 weeks after the last dose of study drug.
Males with female partners of childbearing potential must agree to practice adequate contraceptive methods throughout the study and should avoid conceiving children for 24 weeks following the last dose of study drug.

Exclusion criteria

Prior invasive malignancy (except for non-melanomatous skin cancer) unless disease free for ≥ 3years. (In situ cancer is permissible)
Spinal metastasis and leptomeningeal involvement.
Patients with Bulky Tumors:
- 3 cm in a single dimension (post-surgery)
- Tumor causing uncal herniation or mass effect leading to midline shift with or without symptoms or signs of impending herniation or
- Obstruction to CSF flow
Recurrent or multifocal malignant gliomas.
The patient is not a candidate for cellular therapy as assessed by the study bone marrow transplant physician.
Known immunosuppressive disease or human immunodeficiency virus (HIV) infection.

HIV-positive patients are ineligible due to the unknown safety and efficacy of infusing these patients with CAR T cells genetically modified using retroviral vectors. Additionally, the immunosuppression used for treatment in this study will pose an unacceptable risk.

• Concurrent illness: Patients with active autoimmune disease, documented history of autoimmune disease/syndrome, or any other condition that requires ongoing systemic steroids or systemic immunosuppressive agents, except

Patients with vitiligo or resolved asthma/atopy
Patients with hypothyroidism stable on hormone replacement or Sjogren's syndrome
Patients requiring physiologic doses of corticosteroids (up to 0.5 mg/m2/day dexamethasone equivalent)
- History of or ongoing pneumonitis or significant interstitial lung disease.
- Ongoing or active uncontrolled infection.
- Patients with any clinically significant unrelated systemic illness (serious infections or significant cardiac, pulmonary, hepatic or other organ dysfunction), that in the opinion of the investigator, would compromise the patient's ability to tolerate protocol therapy, put them at additional risk for toxicity or would interfere with the study procedures or results.
- Patients with any of the following cardiac diseases:
New York Heart Association (NYHA) functional class III or IV
Clinically significant cardiac arrhythmia including, but not limited to, Torsade de pointes or requiring a pacemaker
Left ventricular ejection fraction below 50% as determined by echocardiography (ECHO)
- Pregnant or lactating women, due to possible adverse effects on the developing fetus or infant.
- Patients treated on any other therapeutic clinical protocols within 30 days prior to enrollment.
- For females of childbearing potential, a negative serum pregnancy test at enrollment.