Imaging Airway Liquid Absorption in Cystic Fibrosis

University of Pittsburgh

Status: Completed

Conditions: Cystic Fibrosis

Treatments: Other: Absorptive clearance scan

Study type: Interventional

Funder types: Other; NIH

Identifiers

NCT01486199

1R01HL108929-01

R01HL108929 (U.S. NIH Grant/Contract)

Details and patient eligibility

About

The investigators are developing a new nuclear medicine imaging technique for measuring liquid absorption in the airways that can be applied to screen new medications being developed to treat cystic fibrosis (CF). The investigators believe that the absorption of the small molecule radiopharmaceutical Indium 111-diethylenetriaminepentaacetic acid (In-DTPA) will indicate changes in liquid absorption in the airways and demonstrate whether new CF medications will be effective. In this study the investigators will further develop this technique through testing involving pediatric CF patients and healthy control subjects.

Full description

The investigators have recently developed a novel aerosol-based imaging technique to detect changes in liquid absorption in the airways - a central pathophysiological process known to be important in cystic fibrosis (CF) lung disease. This technique may provide a measure of disease severity and indication of therapeutic correction in advance of currently available outcome measures. It involves the simultaneous delivery of two radiopharmaceuticals by inhalation: one an absorbable small-molecule (Indium-111 labeled diethylenetriaminepentaacetic acid; In-DTPA) and the other a non-absorbable particle (Technetium 99m labeled sulfur colloid; Tc-SC). The overarching hypothesis is that In-DTPA absorption provides a quantifiable, non-invasive measurement of airway liquid absorption that (a) is sensitive to CF genotype, (b) uniquely identifies basic disease phenotype and predicts disease severity, and (c) is modulated by therapeutic interventions.

Enrollment: 20 patients

Sex: All

Ages: 6+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Adult healthy control arm:

  • subjects 18 years old or older without a diagnosis of lung disease.

• Pediatric CF arm:

  • subjects 6-14 years with a diagnosis of cystic fibrosis as determined by sweat test or genotype
  • subjects who are clinically stable as determined by the pediatrician co-investigator
  • subjects must have a previously demonstrated ability to perform reproducible pulmonary function testing based on previous clinical visits.

• Females in all groups who are of child-bearing potential will need to have a negative urine pregnancy test.

Exclusion criteria

• Adult healthy control arm:

  • FEV1%p < 80% of predicted
  • nursing mother
  • positive urine pregnancy test or unwilling to test
  • cigarette smoker (regular smoking within 6 months of study).

• Pediatric CF arm:

  • FEV1%p < 40% of predicted
  • nursing mother
  • positive urine pregnancy test for females of childbearing potential
  • unable or unwilling to comply with test procedure
  • cigarette smoker (regular smoking within 6 months of study).
  • Subjects unable to lie recumbent without moving for the 80 minute imaging period will be excluded.

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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20 participants in 2 patient groups

CF pediatric

Experimental group

Description:

In the pediatric arm 10 CF subjects ages 6-14 will perform absorptive clearance scans at baseline and at t=2 years.

Treatment:

Other: Absorptive clearance scan

Controls adult

Experimental group

Description:

In the adult control arm 10 healthy adult subjects will perform a single absorptive clearance scan.

Treatment:

Other: Absorptive clearance scan