Immun Response in Children With MIS-C

University of Cologne

Status: Unknown

Conditions: MIS-C Multisystem Inflammatory Syndrome in Children

Treatments: Diagnostic Test: Analysis of cytokines and immun cell related markers

Study type: Observational

Funder types: Other

Identifiers

NCT05052424

21-1384

Details and patient eligibility

About

Multi-system inflammatory syndrome in children (MIS-C) is a post-viral inflammatory vasculopathy of children and adolescents following Covid-19 infection. It affects one of 1000-5000 children. Latency between mild or asymptomatic Covid-19 infection and MIS-C is 4-6 weeks. The immune response in MIS-C seems to be dysregulated and different from that during acute SARS-CoV-2 infection.

The investigators are planning to investigate the immune response in children with MIS-C.

RNA and protein expression of cytokines and immune cell-related markers will be determined via multiplex ELISA, FACS, quantitative PCR, RNAseq, and Western blot.

The use of highly sensitive multiplex methods will allow for the analysis of a large number of parameters in the smallest possible amount of sample. Sample preparation and analysis will be performed in close collaboration with the Centre for Molecular Medicine Cologne (CMMC) and the Institute of Virology.

With this project the investigators hope to identify risk factors for developing MIS-C and extend the knowledge on therapeutic options for the treatment of this condition.

Enrollment: 20 estimated patients

Sex: All

Ages: Under 18 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Diagnosed with MIS-C (WHO criteria)
• <18 years
• Hospitalized in the Children´s University Hospital of Cologne

Exclusion criteria

• no informed consent

Trial design

20 participants in 1 patient group

Children with MIS-C

Description:

* Children (age \<18 years) hospitalized in the Children´s University Hospital of Cologne diagnosed with MIS-C (WHO criteria) * Assessment of clinical data * Blood samples are taken before therapy and on days 1,2,5,7 und 9. * RNA and protein expression of cytokines and immune cell-related markers will be determined via multiplex ELISA, FACS, quantitative PCR, RNAseq, and Western blot.

Treatment:

Diagnostic Test: Analysis of cytokines and immun cell related markers