Immunoblotting and Goldmann-Witmer Coefficient for Ocular Toxoplasmosis (COPILOT)

Civil Hospices of Lyon

Status: Completed

Conditions: Ocular Toxoplasmosis

Treatments: Procedure: Aqueous humour sampling

Study type: Observational

Funder types: Other

Identifiers

NCT03948750

COPILOT

Details and patient eligibility

About

Ocular toxoplasmosis (OT) is a common cause of posterior uveitis worldwide. The diagnosis of OT is based on clinical findings, but in most cases, laboratory tests are required to confirm the etiology, especially when other diseases are suspected. The aim of this study was to evaluate which methods, between the Goldmann-Witmer coefficient (GWC) and immunoblotting (IB) with both IgG and IgA, in aqueous humour (AH) samples, can be the most sensitive to diagnose OT, in current practice, especially in the first three weeks.

Full description

Goldmann-Witmer coefficient (GWC): compares the levels of intraocular antibody (igG) production to that serum, as measured by ELISA.

GWC = (Level of specific IgG in aqueous humour/level of specific IgG in serum)/(Total IgG in aqueous humour/Total IgG in serum)

0.5 to 2: No intraocular antibody production, 2 to 4: Suggestive of intraocular antibody production, > 4: Diagnostic of intraocular antibody production to a specific microbial pathogen

Enrollment: 87 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients who had underwent aqueous humor and serum sample for ocular toxoplasmosis diagnosis

Exclusion criteria

• Immunocompromised patients

Trial design

87 participants in 2 patient groups

Patients with Ocular Toxoplasmosis

Treatment:

Procedure: Aqueous humour sampling

Patients without Ocular Toxoplasmosis

Treatment:

Procedure: Aqueous humour sampling