Immunosuppressive Effects of Smoking and HIV-1 on the Development of Lung Disease

University of Colorado Denver (CU Denver)

Status: Completed

Conditions: HIV; COPD; Smoking

Study type: Observational

Funder types: Other

Identifiers

NCT02058719

13-2986

Details and patient eligibility

About

This study plans to learn more about pulmonary complications of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS). Even though antiretroviral therapy (ART) has dramatically decreased the number of opportunistic infections and deaths in HIV infected patients, pulmonary complications (including chronic obstructive pulmonary disease (COPD) development and pneumonias resulting in decreased lung function) of HIV/AIDS continue to be a major cause of morbidity and mortality in this population. The mechanisms underlying the increased risk of COPD and decreased lung function in HIV infected individuals is not well understand and needs to be studied.

The investigators hypothesize that the immunoregulatory consequences and immunosuppressive lung milieu secondary to HIV and cigarette smoke combine to increase the risk of lung infection and injury in HIV infected smokers, hastening the development of COPD. The mechanisms will be directly tested using blood and bronchial alveolar lavage (BAL) cells from smokers and nonsmokers with and without HIV infection.

Full description

The first component of this study will be a longitudinal, prospective, 24 weeks study of the effects of HIV-1 infection on innate and acquired immunity in the lung (Cohort A). The second component of this study will be a cross-sectional, case-control study of lung function and immune dysregulation of HIV-1 infected persons on long-term ART (Cohort B).

Cohort A will consist of 120 subjects, stratified by HIV-1 and smoking status

Cohort B will consist of 90 subjects stratified by chronic obstructive pulmonary disease (COPD) and HIV-1 infection.

Enrollment: 210 patients

Sex: All

Ages: 18 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion Criteria (Cohort A):

• Subjects with chronic HIV-1 infection (Cohorts A1 and A2)
• ART naïve or off all ART for >6 months (Cohorts A1 and A2)
• HIV-1 RNA level >1,000 copies/ml (Cohorts A1 and A2)
• HIV-1 seronegative with no high-risk exposure in the past 30 days (Cohorts A3 and A4)
• 18 years and older (All Cohort A)
• Active cigarette smoker (Cohorts A1 and A3)

Inclusion Criteria (Cohort B):

• Age from 30 to 70 years
• Subjects with chronic HIV-1 infection (Cohorts B1 and B2)
• Subjects on stable 3-drug ART regimen with plasma HIV-1 RNA <50 copies/mL for past 6 months (Cohorts B1 and B2)
• HIV-1 seronegative with no high-risk exposure in the past 30 days (Cohort B3) COPD: forced expiratory volume at one second (FEV1)/forced vital capacity (FVC) <70% and forced expiratory volume (FEV), 45-100% of predicted (Cohort B1 and B3)
• Non-COPD: FEV/FVC >70% and an FEV, >80% of predicted (Cohort B2)

Exclusion Criteria (Cohort A and B):

• Pregnancy
• Weight less than 110 pounds (for venipuncture)
• Patient inability to participate in the study and undergo venipuncture and bronchoscopy procedures
• Use of systemic or inhaled corticosteroids in the past 3 months.

Trial design

210 participants in 7 patient groups

Cohort A1

Description:

HIV positive smokers

Cohort A2

Description:

HIV positive non-smokers

Cohort A3

Description:

HIV negative smokers

Cohort A4

Description:

HIV negative non-smokers

Cohort B1

Description:

HIV positive with COPD

Cohort B2

Description:

HIV positive without COPD (non-COPD)

Cohort B3

Description:

HIV negative with COPD