Impact of Dry vs Humidified Culture Conditions on Blastocyst Development and Aneuploidy: A Time-lapse Sibling Oocyte Study

ART Fertility Clinics LLC

Status: Not yet enrolling

Conditions: Incubator Stay; Fertilization Rate; Ploidy Rate; Biopsy Rate; ICSI; Blastocyst Quality; Total Blastulation

Treatments: Device: K- system incubator

Study type: Interventional

Funder types: Other

Identifiers

NCT07270250

2504-ABU-013-LV

Details and patient eligibility

About

IVF incubators are essential for maintaining the micro-environment required for embryo development. Incubator technology has progressed from early humidified box systems to benchtop and now time-lapse platforms, driving the development of dry incubator chambers. Both humidified and dry systems have specific pros and cons. Evidence to date suggests that humidified chambers may support better blastocyst development in certain "no-refresh" continuous culture conditions, but current data are limited and study designs remain weak. This study aims to compare sibling oocytes cultured in dry versus humidified chambers within a GERI time-lapse incubator under continuous culture conditions, assessing effects on viability and developmental outcomes. Findings may inform optimal incubation strategies to improve IVF success rates while supporting uninterrupted workflows and potentially improving cost-benefit efficiency in the IVF laboratory.

Enrollment: 60 estimated patients

Sex: Female

Ages: 18 to 43 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• PGT-A cycles with more than at least 4 mature oocytes
• ICSI
• maternal age 18- 43 years old
• PGT-A intended cycles with trophectoderm biopsies on day 5 /6/7
• patients with more than 8 oocytes expected for ICSI
• BMI <35
• fresh and frozen ejaculated sperm

Exclusion criteria

• PGT-M /PGT-SR cycles
• fresh and frozen testicular sperm
• IVF insemination
• previous history of fertilization failure

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Sequential Assignment

Masking: None (Open label)

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60 participants in 2 patient groups

Dry Chamber of Geri

Other group

Treatment:

Device: K- system incubator

Humidified Chamber of Geri

Other group

Treatment:

Device: K- system incubator