Impact of Mutations in Aminoacyl TRNA Synthetases on Protein Translation and Cellular Stress (FIBROMARS)

Assistance Publique - Hôpitaux de Paris

Status: Not yet enrolling

Conditions: Infantile Liver Failure Syndrome 1; Rajab Interstitial Lung Disease with Brain Calcifications 2; Interstitial Lung and Liver Disease; Neurologic, Endocrine and Pancreatic Disease, Multisystem, Infantile-Onset 2

Treatments: Other: Skin biopsy

Study type: Interventional

Funder types: Other

Identifiers

NCT05514470

2024-A01311-46 (Other Identifier)

APHP220276

Details and patient eligibility

About

Mutations in the genes encoding cytosolic aminoacyl-tRNA synthetases are responsible for early-onset multisystemic diseases including to varying degrees interstitial lung disease, liver damage, neurological and digestive disorders, and systemic inflammation. These are rare and severe diseases whose pathophysiology is poorly understood.

The investigative team hypothesizes that mutations within these genes are responsible for a decrease in protein translation and lead to a cellular stress response similar to that induced by amino acid deprivation. The investigative team also hypothesizes that these alterations could be corrected by high-dose supplementation in the culture medium of the corresponding amino acid.

The main objective of the study is to precisely determine the consequences of cytosolic aminoacyl-tRNA synthetase mutations at the cell level on protein translation.

Full description

Mutations in the genes encoding cytosolic aminoacyl-tRNA synthetases are responsible for early-onset multisystemic diseases including to varying degrees interstitial lung disease, liver damage, neurological and digestive disorders, and systemic inflammation. These are rare and severe diseases whose pathophysiology is poorly understood.

The investigative team hypothesizes that mutations within these genes are responsible for a decrease in protein translation and lead to a cellular stress response similar to that induced by amino acid deprivation. The investigative team also hypothesizes that these alterations could be corrected by high-dose supplementation in the culture medium of the corresponding amino acid.

The main objective of the study is to precisely determine the consequences of cytosolic aminoacyl-tRNA synthetase mutations at the cell level on protein translation.

The parameters below will be studied in vitro in cell culture from skin biopsies of patients and control cells:

• Determination of total protein content
• The incorporation of d-methionine, leucine, tyrosine or phenylalanine into proteins
• The study of polysomes profiling
• The study of the assembly of the ribosomal 43S pre-initiation complex
• The phosphorylation of eIF2α and 4EBP and the expression of ATF4
• Ribosome profiling
• Transfer RNA (tRNA) sequencing
• The production of reactive oxygen species (ROS)

The results of these studies will be compared:

• Between patient cells and control cells
• Between genetically corrected patient cells, by stable transfection of the wild-type cDNA of the concerned genes and uncorrected cells
• Between patient cells cultured in medium enriched with the corresponding amino acid.

Enrollment: 24 estimated patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients carrying mutations in genes encoding cytosolic aminoacyl-tRNA synthetases responsible for a multi-systemic phenotype
• Information and consent of the patient if an adult and of the holders of parental authority if a minor patient and of the minor patient

Exclusion criteria

• Non-consent of one of the holders of parental authority or of the minor patient or of adult patient

Contrôl patients :

• Fibroblasts from control patients without mutation in genes encoding cytosolic aminoacyl-tRNA synthetases, from an existing biological collection. The control patients will be selected according to the age at which the skin biopsy was performed in order to have an age match between the patients and the controls.
• Information and consent of the patient if an adult and of the holders of parental authority if a minor patient and of the minor patient

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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24 participants in 1 patient group

Patients

Experimental group

Description:

Patients with mutations in genes encoding cytosolic aminoacyl-tRNA synthetases and cared at Necker Hospital.

Treatment:

Other: Skin biopsy