Impact of Probiotics in Modulation of Intestinal Microbiota

Maria Isabel Toulson Davisson Correia

Status: Completed

Conditions: Colorectal Cancer

Treatments: Dietary Supplement: Saccharomyces boulardii

Study type: Interventional

Funder types: Other

Identifiers

NCT01895530

ETIC 0391.0.203.000-09

Details and patient eligibility

About

The investigators would study about impact of the administration of probiotics in the intestinal mucosa of patients undergoing resection colic, by evaluating cytokine profile by quantitative real time PCR. The investigators believe that patients who use probiotic preoperative would provide cytokine profile less inflammatory than those of the control group.

Full description

Samples of colorectal cancer-free mucosa derived from patients who underwent surgical resection were investigated. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to determine expression levels of the following genes: IL10, IL1B, IL23A, TNF, IL12B, INFG, IL17A.

Enrollment: 33 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• if he or she were older than 18 years
• intended to undergo elective CRC resection at UFMG Hospital

Exclusion criteria

• Patients were excluded if they were not able to receive the probiotics seven days before the operation
• if colon resection was not performed due to changes in operation strategy, or if they discontinued probiotic use on their own or
• if they removed their consent.

Trial design

Primary purpose: Health Services Research

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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33 participants in 2 patient groups

Control group

No Intervention group

Description:

Conventional treatment with no probiotic supplementation

Probiotic group

Experimental group

Description:

The patients received one oral lyophilized yeast capsule, each of which contains 100 mg (0,5 x 109 cfu/g) of Saccharomyces boulardii (Merck S.A., Biocodex, Beauvais, French), once a day. The treatment started at least seven days before surgery and stopped on the operation day.

Treatment:

Dietary Supplement: Saccharomyces boulardii