Impacts of Nitrogen Deposition in the Natural Environment on Pollen Allergy in Belgium (NITROPOL-BE)

prof. dr. Rik Schrijvers

Status: Enrolling

Conditions: Pollen Allergy

Treatments: Diagnostic Test: Immunoreactivity testing; Diagnostic Test: Immunoreactivity testing (negative control)

Study type: Interventional

Funder types: Other

Identifiers

NCT06714149

B2/212/P1/NITROPOL-BE (Other Grant/Funding Number)

S65184

Details and patient eligibility

About

The investigators want to study the effect of environmental nitrogen enrichment on the allergy burden in pollen-sensitized subjects.

Full description

The investigators want to study allergen-specific IgE reactivity from a cross-sectional sample of pollen-allergic patients (and negative controls) against environmental and experimental pollen samples. Following aspects will be compared:

• To study the basophil degranulation with different amounts of pollen on fresh patient-derived basophils, to determine the concentration of pollen needed to degranulate 5% (EC5), and 50% (EC50) of basophils.
• To study he overall intensity (quantitative), as well as IgE-mediated hyper- or hyporecognition of different fragments in the pollen samples (qualitative) in a subset of patients.
• To associate allergic rhinoconjunctivitis and food-pollen syndrome related symptoms with reactivity to different pollen samples.

Enrollment: 200 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Positive skin prick test for grass and/or birch pollen

Exclusion criteria

• Desensitization therapy during the last two years

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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200 participants in 2 patient groups

Allergic subjects

Other group

Description:

All subject with positive skin prick testing for grass and/or birch pollen are assigned to this group.

Treatment:

Diagnostic Test: Immunoreactivity testing

Healthy controls

Other group

Description:

Subjects with negative skin prick testing for grass and/or birch pollen are assigned to this group and used for as negative controls in our experiments.

Treatment:

Diagnostic Test: Immunoreactivity testing (negative control)