Implantation Rate of Vitrified-warmed Collapsed Blastocysts

Centre for Reproductive Medicine, Belgium

Status: Completed

Conditions: Implantation Rate of Vitrified Blastocysts

Treatments: Procedure: Collapse

Study type: Interventional

Funder types: Other

Identifiers

NCT01980225

VanLanduyt2013

Details and patient eligibility

About

In our centre, the implantation rate of frozen blastocysts (cryopreserved using vitrification)was 20,3% (Van Landuyt et al. 2011). This implantation rate is lower when compared to data from other observational studies. Retrospective studies have shown that artificial shrinkage of the blastocoelic cavity (also called collapse) and dehydration of the blastocyst just before vitrification can have a positive effect on the survival after warming. This collapse can be induced by using a laser. The study of Iwayama et al. 2010 has shown a similar survival rate but an increase in the implantation rate of collapsed blastocysts compared to non-collapsed blastocysts.

The aim of the study is to investigate the effect of artificial shrinkage by laser-induced collapse on the implantation potential of vitrified-warmed day 5 or day 6 blastocyst stage embryos in a prospective randomised controlled trial.

Enrollment: 270 patients

Sex: Female

Ages: 18 to 38 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• female age <39 years
• at least 1 blastocyst available for cryopreservation

Exclusion criteria

• sperm origin: testicular or epididymal sperm extraction
• IVM (in-vitro maturation) cycles
• PGD cycles (preimplantation genetic diagnosis)

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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270 participants in 2 patient groups

Collapse

Experimental group

Description:

This arm includes the patients where laser-induced collapse (=artificial shrinkage) of all blastocysts is performed before vitrification

Treatment:

Procedure: Collapse

control

No Intervention group

Description:

This control arm includes the patients of which the blastocysts are vitrified without performing collapse