Improving Embryogenesis by Adding Growth Factors to In Vitro Human Culture Media (GFSSSCM)

Ibn Sina Hospital

Status: Completed

Conditions: In Vitro Human Embryo Development

Treatments: Other: Growth Factors and Cytokine adding to embryo culture media; Other: No Intervention

Study type: Interventional

Funder types: Other

Identifiers

NCT02426008

IbnSina IVF

Details and patient eligibility

About

The investigators suppose that adding growth factors that had been found in the female tubal fluid and uterus to In Vitro single step culture media for human embryos might improve the the embryogenesis In Vitro and the clinical outcome of the resulting off spring.

Full description

The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding growth factors ( Insulin-like growth factor I, Insulin-like growth factor II, transforming growth factor-beta and epidermal growth factor ) as a promoting growth factors to Lifeglobal culture media might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between to arm of the same culture media and the same culture condition but with one arm with growth factors and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.

Enrollment: 426 patients

Sex: Female

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• all patients

Exclusion criteria

• over 40 years old

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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426 participants in 2 patient groups, including a placebo group

Culture media with growth factors and Cytokine

Experimental group

Description:

Growth factors and Cytokine adding to culture media to detect the deference and monitor the embryological outcome.

Treatment:

Other: Growth Factors and Cytokine adding to embryo culture media

In Vitro culture media

Placebo Comparator group

Description:

culture media to monitor the embryological outcome as control group

Treatment:

Other: No Intervention