In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines (invitro)

Meenakshi Ammal Dental College and Hospital

Status: Completed

Conditions: Apoptosis

Treatments: Other: mangosteen extract

Study type: Interventional

Funder types: Other

Identifiers

NCT03728192

MAHER-MU-004-IEC/2016

Details and patient eligibility

About

The present study is an effort to investigate the hypothesis that in-vitro vitality and antiapoptotic effect of alcoholic crude extract of mangosteen on Oral cancer( H357) cell lines and Cevical cancer (HeLa) cell lines.

Full description

The oral and cervical cells were investigated by MTT (3-4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, DNA fragmentation detection by TUNEL (Terminal deoxynucleotidyl transferase-mediated d-UTP Nick End Labeling) assay and Apototic assay using Annexin V/FITC Kit.

Enrollment: 2 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients with oral squamous cell carcinoma
• Patients with cervical carcinoma

Exclusion criteria

• Patients with leukaemia

Trial design

Primary purpose: Prevention

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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2 participants in 2 patient groups

Mangosteen treated group

Experimental group

Description:

HeLa and H357 cell lines were procured and were further subdivided into 2 subdivisions and were assigned interventions: Mangosteen group- cells treated with mangosteen extract and Camptothecin group - cells treated with standard anticancer drug camptothecin(25 micro mole)

Treatment:

Other: mangosteen extract

Untreated group

No Intervention group

Description:

H357 and HeLa cell line without any drug intervention.