In Vitro Maturation Induction of Oocytes From a Woman With Syndrome of Repeated Oocyte Maturation Failure in Vitro

Kunming Medical University

Status

Completed

Conditions

Oocyte Maturation Failure

Treatments

Other: no intervention

Study type

Observational

Funder types

Other

Identifiers

NCT01977664

201301

Details and patient eligibility

About

Donated oocytes is from a patient with repeated oocyte maturation failure in IVF cycles. Cytoplasm of matured metaphase II oocyte and active MPF were injected into the donated oocytes.

Full description

The study reported a woman with syndrome of repeated oocyte maturation failure where there is repeated production of immature oocytes.She underwent two IVF cycles in other hospital. All oocytes obtained were immature and the treatment were stopped.She underwent her third try in our hospital. The couple gave their informed consent: ICSI will be performed if maturation occurred, otherwise, the treatment would be stopped and the oocytes would be donated for study if the oocytes were not matured after 24 hours of culture. After ovarian hyperstimulation,14 oocytes were obtained. None of them was matured. The cycle was canceled and the oocytes were donated for study.To induce the immature oocytes in vitro, oocytes were 1)cultured in follicular fluid containing matured metaphase II oocyte; 2) performed intracytoplasmic sperm injection (ICSI) to induce intracellular Ca2+]i oscillations; 3)injected with active maturation promoting factor (MPF), 4)injected with cytoplasm of matured metaphase II oocyte.

Enrollment

1 patient

Sex

Female

Ages

32 to 32 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Clinical diagnosis of syndrome of repeated oocyte maturation

Exclusion criteria

patients with matured oocytes in IVF cycles