In Vivo and in Vitro Anabolic Potential of Essential Amino Acids Following Resistance Exercise (DiEx)

University of Toronto

Status: Active, not recruiting

Conditions: Interventional

Treatments: Dietary Supplement: BCAA Supplement; Dietary Supplement: Collagen Supplement; Dietary Supplement: Di-Leucine Supplement

Study type: Interventional

Funder types: Other

Identifiers

NCT05754125

DiEx

Details and patient eligibility

About

This study seeks to investigate the anabolic potential of a dileucine-enriched essential amino acid (EAA) formulation compared with a branched chain amino acid (BCAA) alternative and a collagen beverage on muscle protein anabolism and catabolism following a bout of resistance exercise training. To do this, investigators will employ a novel 'breath test' method developed in our laboratory as well as blood and urine sampling. The results of this study will allow us to better understand the anabolic potential of dileucine which could have implications for people engaging in regular resistance training (such as athletes) as well as people that need to preserve muscle mass (older people who are susceptible to anabolic resistance and sarcopenia, or muscle wasting).

Enrollment: 12 patients

Sex: All

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Trained individuals currently performing structured exercise (e.g., running, weightlifting, team-sport activity) at least 2 days per week for the previous 3 months

Exclusion criteria

• Inability to perform physical activity as determined by the PAR-Q
• Inability to adhere to protocol guidelines (e.g., alcohol, habitual diet)
• Regular tobacco use
• Illicit drug use (e.g., growth hormone, testosterone, etc.)
• Diagnosed medical condition under the care of a physician (e.g. type 2 diabetes)
• Inability to abstain from supplements (e.g. protein, creatine, HMB, BCAA, phosphatidic acid, etc.) at least three weeks before the trial
• Individuals on any medications known to affect protein metabolism (e.g., corticosteroids, non-steroidal anti-inflammatories, or prescription-strength acne medications)
• Participants that are amenorrheic (females only)
• On medications that may interfere with protein metabolism (e.g. anti-inflammatory drugs, hormone therapy)
• Regular tobacco use (e.g. daily use of oral or inhaled tobacco)
• Illicit drug use (e.g. growth hormone, testosterone, etc.)
• Inability to comply with the study protocol as judged by the investigators

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Triple Blind

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12 participants in 3 patient groups

Di-Leucine supplement

Experimental group

Description:

Participants will consume a amino acid supplement containing Di-Leucine following resistance exercise and their whole body anabolism will be determined over the subsequent 6 hours

Treatment:

Dietary Supplement: Di-Leucine Supplement

BCAA Supplement

Experimental group

Description:

Participants will consume a amino acid supplement containing branched chain amino acids (BCAA) following resistance exercise and their whole body anabolism will be determined over the subsequent hours

Treatment:

Dietary Supplement: BCAA Supplement

Collagen Supplement

Experimental group

Description:

Participants will consume a amino acid supplement containing collagen protein following resistance exercise and their whole body anabolism will be determined over the subsequent hours

Treatment:

Dietary Supplement: Collagen Supplement