Induction of Neutrophilic Traps ( NETs ) by the Environment and Infectious Inflammatory Lung Cystic Fibrosis (MucoNETs)

Regional University Hospital Center (CHRU)

Status: Completed

Conditions: Cystic Fibrosis

Study type: Observational

Funder types: Other

Identifiers

NCT02564393

PHAO09-PD / MucoNETs

Details and patient eligibility

About

The study is defined in 3 stages of in vitro assays :

Phase 1 : Secretion of NETs in response to lung disease and their secretion of factors.

This is a transversal study in which we compare :

• Producing NETs (DNA and proteases ) by neutrophils purified from sputum and blood of patients
• Producing NETs (DNA and proteases ) by purified neutrophils in the blood of patients and blood of control subjects

Phase 2: Mechanisms of activation of NETs secretory pathways . This phase will identify receptors (TLR ) involved in the secretion of NETs and analyze the involvement of dependent transduction pathways calcium and PKC . It will be performed on control blood neutrophils.

Phase 3: Analysis of cell death ( NETose )

This is a transversal study that will analyze :

the mode of cell death of neutrophils purified blood and sputum of patients and control blood neutrophils by flow cytometry and transmission electron microscopy .

Enrollment: 55 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

Patients :

• Men or women over 18
• Patients with mucoviscidose in stable condition, i.e. not having presented an acute push of bronchopulmonary attack requiring a antibiothérapie or a hospitalization for treatment of its disease during the 2 previous weeks
• Signed consent

Volunteers :

• Men or women over 18
• Without respiratory antecedent nor known respiratory pathology
• Signed consent

Exclusion criteria

Patients :

• In exclusion period of an other study

Volunteers :

• In exclusion period of an other study
• Antibiotic treatment and/or anti-inflammatory drug (AINS or corticoids) in the 2 weeks before inclusion in the study

Trial design

55 participants in 2 patient groups

T

Description:

Control

A

Description:

Adult with cystic fibrosis