Inflammation, Intracellular Invasion and Colonization of the Nasal Mucosa by Staphylococcus Aureus (I3COSa)

Centre Hospitalier Universitaire de Saint Etienne

Status: Completed

Conditions: Healthy

Treatments: Diagnostic Test: nasal swab

Study type: Interventional

Funder types: Other

Identifiers

NCT04469348

19CH050

2020-A00321-38 (Other Identifier)

Details and patient eligibility

About

An inflammatory state of the nasal cells (very close to keratinocytes) could favour the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium.

Staphylococcus aureus is a commensal bacterium of the skin and mucous membranes that colonizes approximately 2 billion people worldwide Staphylococcus aureus is also a leading cause of community and healthcare-associated infection. Staphylococcus aureus has demonstrated its ability to invade many non-professional phagocytic cell lines such as keratinocytes, osteoblasts, fibroblasts, epithelial cells and endothelial cells. During pro-inflammatory stimulation, internalization of Staphylococcus aureus into keratinocytes is mainly mediated by ICAM-1. These results suggest that, in humans, an inflammatory state of the nasal cells (very close to keratinocytes) could promote the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium.

Full description

The main objective is to compare the expression level of ICAM-1 in nasal cells with and without the presence of Staphylococcus aureus.

Enrollment: 157 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Subject affiliated to a social security scheme or entitled person
• Subject who received informed information about the study et signed consent
• Adult subject (≥18ans)
• Healthy Volunteer

Exclusion criteria

• Subject undergoing antibiotic treatment or having received antibiotic treatment (oral, IV or local) in the month prior to inclusion
• Subject with a major haemostasis disorder or anticoagulant treatment contraindicated for surgery
• Subject with a pathology increasing the nasal carriage of S. aureus (chronic furunculosis, diabetes, HIV infection, chronic rhinosinusitis)
• Subject under guardianship or trusteeship
• Pregnant woman

Trial design

Primary purpose: Diagnostic

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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157 participants in 1 patient group

Healthy volunteers

Experimental group

Description:

Healthy volunteers will be included. They will have nasal swab at the inclusion visit to detect contamination of S. Aureus. If contamination of S. Aureus: they will have 12 follow-up visits (1 per month) If no contamination of S. Aureus: their participation stops

Treatment:

Diagnostic Test: nasal swab