Inflammation, Platelets and Sickle Cell Disease (Il-Padre)

Toulouse University Hospital

Status: Enrolling

Conditions: Platelet Activation; Sickle Cell Disease; Thromboinflammation

Treatments: Biological: Blood sampling

Study type: Observational

Funder types: Other

Identifiers

NCT05646888

RC31/22/0255

Details and patient eligibility

About

Sickle cell disease (SCD) is an autosomal recessive genetic disorder linked to a single mutation on beta-globin chains. This leads to red blood cell deformation and chronic hemolysis which can result in vaso-occlusive events, anemia and vasculopathy. Pathophysiology is incompletely understood, and beyond red blood cell's abnormalities this involves hemostasis and innate immunity. The aim of our study is to describe the mechanisms of thrombo-inflammation during the vaso-occlusive crisis (VOC) in adults with sickle cell disease.

Full description

Pathophysiology of sickle cell disease is incompletely understood. The typical change of red blood cells into sickle cells lead to post-capillary stream abnormalities. This phenomenon is responsible of ischemia-reperfusion injuries. Chronic hemolysis is the second part of the pathophysiology. The consequences are vasoconstriction, endothelial lesions, chronic inflammation, hemostasis and platelets activation. Thrombo-inflammation concept was proposed by Tanguay to describe the interactions between hemostasis, platelets and innate immune cells (neutrophils polynuclear) during thrombotic process. Since this time, this concept was largely described in many clinical situations such as septicemia, COVID-19, coronaropathy, auto-immune diseases and sickle cell disease.

In this project, we will study platelets activation and thrombo-inflammation markers in the beginning of a vaso-occlusive crisis, during the crisis and two months after the crisis. Blood samples will be collected during a routine care sample.

Biological markers studied will be:

• Soluble and surface markers of platelet activation; platelet-leukocytes aggregates
• Plasmatic eicosanoids produced by platelets (TXB2) and immune cells
• In vitro platelets reactivity; dynamic thrombus formation in normal and pathologic arterial blood stream
• Platelet's inflammasome

Enrollment: 25 estimated patients

Sex: All

Ages: 18 to 99 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patient with sickle cell disease diagnosis, hospitalized in emergency department and/or internal medicine department
• Patient older than 18 years
• Written consent to participate to the study
• Patient with health insurance
• Patient able to receive information about the study

Exclusion criteria

• Age < 18 years
• Non consent to participate to the study
• Women in pregnancy or breastfeeding
• Treatment with aspirin or non steroidal anti inflammatory drug
• Protected patient
• Patient already involved in a study requiring collection of additional biological samples