Inflammation-Related Endothelial Barrier Dysfunction in Patients With Apical Periodontitis

University of Turin

Status: Completed

Conditions: Apical Periodontitis; Healhty

Treatments: Other: Apical lesions cure

Study type: Observational

Funder types: Other

Identifiers

NCT07292363

0094485/II

Details and patient eligibility

About

Inflammation is a common factor in chronic periodontitis and systemic diseases. However, to date, there is no scientific evidence supporting a causal effect of inflammation caused by apical periodontitis on endothelial barrier dysfunction.

A case-control study was designed to evaluate serum levels of endothelial barrier dysfunction factors in a sample of healthy patients aged 25 to 55, with or without apical periodontitis, before endodontic treatment and 6 and 12 months after treatment.

The aim of this study is to investigate the potential relationship between the presence of chronic endodontic lesions and inflammation-related alterations of the endothelial barrier that may compromise its integrity, as well as to determine whether endodontic treatment can reduce these factors, thereby preventing changes in endothelial permeability.

Full description

Systemically healthy, informed, and consenting patients of both sexes under 55 years of age with a diagnosis of apical periodontitis who undergo endodontic treatment will be enrolled as cases and monitored at 6 and 12 months after treatment. Systemically healthy, informed, and consenting patients of both sexes under 55 years of age without apical periodontitis will be enrolled as control subjects.

The study has been designed to assess serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8), as well as the endothelial junctional proteins zonula occludens-1 (ZO-1), claudin-5, and vascular endothelial cadherin (VE-cadherin), using enzyme-linked immunosorbent assay (ELISA).

Enrollment: 45 patients

Sex: All

Ages: 25 to 55 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• 25-55 years
• in general health
• with or without endodontic disease

Exclusion criteria

• under 25 years
• over 55 years
• subjects with systemic or oral diseases other than endodontic affections
• patients who underwent other dental treatments in the previous 6 months

Trial design

45 participants in 2 patient groups

AP group

Description:

Healthy subjects with apical periodontitis between 25-55 years

Treatment:

Other: Apical lesions cure

Control Group

Description:

Healthy subjects without apical periodontitis between 25-55 years