Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein (ICADP)

Bispebjerg Hospital

Status: Completed

Conditions: Muscle Protein Synthesis; Amino Acid

Treatments: Dietary Supplement: Placebo; Dietary Supplement: Cluster Dextrin; Dietary Supplement: Meat Hydrolysate

Study type: Interventional

Funder types: Other

Identifiers

NCT03303729

H-17017363

Details and patient eligibility

About

10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin.

The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin.

Hypotheses:

I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period.

II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.

Enrollment: 10 patients

Sex: Male

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• young healthy adults,
• organised sport/training 1-3 times per week

Exclusion criteria

• Subject younger than 18 years or above 30 years
• BMI > 30
• Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus.
• Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week.
• Participating in systematized exercise more than 3 times per week.
• Vegetarian.
• Participating in a special diet which deviates from the standard of young adults.

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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10 participants in 2 patient groups

Meat hydrolysate & Cluster Dextrin

Experimental group

Description:

Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin.

Treatment:

Dietary Supplement: Meat Hydrolysate

Dietary Supplement: Cluster Dextrin

Meat hydrolysate & placebo

Active Comparator group

Description:

Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose.

Treatment:

Dietary Supplement: Meat Hydrolysate

Dietary Supplement: Placebo