Influence of Dietary Fiber-rich Meals on Gene Expression and Postprandial Glucose and Lipid Response

Lund University

Status: Completed

Conditions: Hypoglycemia; Hyperglycemia

Treatments: Other: Mixture of fiber; Other: Rye bran; Other: Oat bran; Other: Control; Other: Sugar beet fiber; Other: Spray-dried oat drink

Study type: Interventional

Funder types: Other

Identifiers

NCT01005342

KCROS07

Details and patient eligibility

About

The aim of this study is to

• Measure the effect on gene expression in leukocytes from a meal rich in oat bran
• Investigate the postprandial glucose, insulin and triglyceride responses after intake of meals containing fiber from different sources (oat, rye and sugar beet fiber) or a meal containing a mixture of these three fibers

Full description

Dietary fiber has long been known to give beneficial health effects. Yet, the understanding of how fiber-rich meals regulate molecular events at a gene level is limited. Also, few studies have compared the effects of different fiber sources on postprandial responses and hardly any study the effects of fiber mixtures in the same meal, even though this is more similar to regular eating habits.

Healthy subjects will come to the study center after an overnight fast, to ingest breakfasts randomly enriched with different fiber. The meals contains either spray-dried oat drink, rye bran, sugar beet fiber a mixture of these three fibers, oat bran or no added fiber (control). All meals are adjusted to contain the same total amount of available carbohydrates and fat. Blood leukocytes for gene expression profiling were taken before and 2 h after consumption while blood samples for analysis of postprandial glucose, insulin and triglyceride levels were taken every 30 min during 3 h.

NuGO Affymetrix Human Genechip NuGO_Hs1a520180 are used for the microarray analysis and analysis is performed with linear mixed models and enrichment analysis to identify functional gene sets that responded to the specific oat bran effect.

Enrollment: 18 patients

Sex: All

Ages: 20 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• BMI 18-30

Exclusion criteria

• pregnancy
• breastfeeding
• diabetes mellitus
• hepatitis B
• blood lipid lowering pharmaceuticals
• intolerance or allergy to cereals or sugar beet fiber

Trial design

18 participants in 6 patient groups, including a placebo group

Mixture of fiber

Experimental group

Description:

Single intake of a mixture of spray-dried oat drink, rye bran and sugar beet fiber

Treatment:

Other: Mixture of fiber

Sugar beet fiber

Experimental group

Description:

Single intake of sugar beet fiber

Treatment:

Other: Sugar beet fiber

Rye bran

Experimental group

Description:

Single intake of rye bran

Treatment:

Other: Rye bran

Oat bran

Experimental group

Description:

Single intake of oat bran

Treatment:

Other: Oat bran

Spray-dried oat drink

Experimental group

Description:

Single intake of spray-dried oat drink

Treatment:

Other: Spray-dried oat drink

Control

Placebo Comparator group

Description:

Single intake of a meal with no added fiber

Treatment:

Other: Control