Influence of Leucine Enriched Amino Acids on Myofibrillar and Collagen Protein Synthesis

Daniel Moore

Status: Active, not recruiting

Conditions: Interventional

Treatments: Dietary Supplement: Leucine enriched amino acids; Dietary Supplement: Carbohydrate Placebo

Study type: Interventional

Funder types: Other

Identifiers

NCT05554653

ULE

Details and patient eligibility

About

The primary objective will be to determine the effect of leucine-enriched essential amino acids (LEAA) compared to carbohydrate placebo on dietary incorporation of [D5] Phenylalanine & [D5] Glycine into the three skeletal muscle protein pools (myofibrillar, sarcoplasmic and collagen), both following resistance exercise and at rest, with the two tracers provided as a 'intrinsically labeled' bolus. Other outcomes will relate to molecular regulation of protein synthesis.

Enrollment: 8 patients

Sex: All

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Healthy, male and female, recreationally-active participants.
• Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix B).
• Self-reported not having engaged in resistance exercise and/or lower body plyometrics for at least 3 months prior to the study.
• Participants will be aged 18-35 years old.
• Participants are willing to abide by the compliance rules of this study.
• Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).

Exclusion criteria

• Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor.
• Self-reported regular tobacco use.
• Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
• Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
• Use of birth control and discontinued use within the last 3 months (female participants).

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Triple Blind

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8 participants in 2 patient groups, including a placebo group

Leucine enriched essential amino acids

Active Comparator group

Description:

Leucine enriched essential amino acids (4g total, of which 1.6g are leucine). 20g carbohydrate, 2g of protein free drink powder, and 5g of Splenda to mask taste.

Treatment:

Dietary Supplement: Leucine enriched amino acids

Carbohdyrate Placebo

Placebo Comparator group

Description:

Iso-caloric placebo (4g maltodextrin to replace LEAA). 20g carbohydrate, 2g of protein free drink powder, and 5g of Splenda to mask taste.

Treatment:

Dietary Supplement: Carbohydrate Placebo