Influence of Protein Hydrolysis on Dietary Protein Digestibility and Metabolism in Healthy Subjects

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Status: Completed

Conditions: Healthy

Treatments: Dietary Supplement: 15N-labelled intact casein; Dietary Supplement: mixture of AA; Dietary Supplement: 15N-labelled hydrolyzed casein; Other: Intestinal and metabolic exploration

Study type: Interventional

Funder types: Other

Identifiers

NCT00873951

DEGLAIRE CT

Details and patient eligibility

About

Hydrolysis of dietary protein may impact their gastrointestinal kinetics and further metabolism. The primary goal of this work was to measure the metabolic fate of intact or hydrolyzed protein using a protein of good nutritional quality, i.e., casein. A second aim of this study was to assess the true ileal digestibility of dietary protein using different methods including the hydrolyzed casein method and to measure its possible effect on endogenous intestinal N losses in humans.

Enrollment: 26 patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• healthy subjects
• BMI between 18 and 25

Exclusion criteria

• allergy to dairy proteins
• positive serology to AgHbS, HCV or HIV
• pregnancy
• abusive drug or alcohol consumption.

Trial design

26 participants in 3 patient groups

Intact casein

Experimental group

Description:

Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled intact casein

Treatment:

Other: Intestinal and metabolic exploration

Dietary Supplement: 15N-labelled intact casein

Hydrolyzed casein

Experimental group

Description:

Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein

Treatment:

Dietary Supplement: 15N-labelled hydrolyzed casein

Other: Intestinal and metabolic exploration

AA

Experimental group

Description:

Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine

Treatment:

Other: Intestinal and metabolic exploration

Dietary Supplement: mixture of AA