Insulinotropic Effect of GIP and GLP-1 Before and After Reduced Glucose Tolerance

Glostrup University Hospital, Copenhagen

Status: Completed

Conditions: Deteriorated Glucose Homeostasis; Insulin Resistance; Inactivity

Treatments: Other: No intervention; Behavioral: Steroid hormone

Study type: Interventional

Funder types: Other

Identifiers

NCT01173978

DYS-CLAMP

Details and patient eligibility

About

The incretin effect in patients with type two diabetes is reduced. The investigators have previously shown that it is possible to induce a defect in the incretin effect in healthy individuals. The purpose of this study is to evaluate the insulinotropic affect of the incretin hormones in healthy individuals before and after a deterioration of the glucose homeostasis.

Full description

The incretin effect in patients with type two diabetes is reduced. The investigators have previously shown that it is possible to induce a defect in the incretin effect in healthy individuals. The purpose of this study is to evaluate the insulinotropic affect of the incretin hormones in healthy individuals before and after a deterioration of the glucose homeostasis. The evaluation is done by infusing GIP, GLP-1 or saline during hyperglycemic clamps.

Enrollment: 10 patients

Sex: Male

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Caucasians without type 2 diabetes mellitus
• Normal OGTT (75 g of glucose) according to WHO's criteria
• BMI 20-30
• Hemoglobin > 8.0 mmol/l
• Informed consent

Exclusion criteria

• Liver disease (ALAT > 2 x normal level)
• Nephropathy (s-creatinin > 130 µM or albuminuria)
• Relatives (parents/siblings) with T2DM
• Medical treatment witch cannot be stopped for 12 hours

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Single Group Assignment

Masking: Single Blind

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10 participants in 2 patient groups

No intervention

Experimental group

Description:

Each participant is examined during a normal, active lifestyle, without any intervention: The examinations include an OGTT, a glucagon test, three hyperglycemic clamps with infusion of a)GLP-1; b)GIP; c)Nacl

Treatment:

Other: No intervention

Intervention

Experimental group

Description:

Each participant is examined during a 12 days intervention.The examinations include an OGTT, a glucagon test, three hyperglycemic clamps with infusion of a)GLP-1; b)GIP; c)Nacl

Treatment:

Behavioral: Steroid hormone