Interaction Between Immune Cells and Bacteria Associated With Periodontitis

University of Copenhagen

Status: Completed

Conditions: Inflammation; Aggressive Periodontitis; Periodontal Diseases; Microbial Disease; Generalized Chronic Periodontitis; Inflammation Gum; Dysbiosis; Periodontitis; Generalized Aggressive Periodontitis; Periodontal Pocket; Chronic Periodontitis; Immunologic Disease; Rheumatoid Arthritis

Treatments: Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria; Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers; Diagnostic Test: periodontitis-associated bacteria presence; Genetic: Analysis of selected single nucleotide polymorphisms (SNPs)

Study type: Observational

Funder types: Other

Identifiers

NCT03225950

H-16024734

Details and patient eligibility

About

This study evaluates the interaction between host immune cells and bacteria associated with periodontitis. It comprises biological material from donors with and without periodontal disease. Specifically, we collect a spit and blood sample to conduct in vitro stimulations and measurements of selected parameters related to periodontitis to clarify obscure areas in the immunologic pathogenesis of this disease.

Full description

Periodontitis is a prevalent, multifactorial inflammatory disease characterized by the interaction between microorganisms organized in biofilms on tooth surfaces and host immune cells, leading to an inflammatory destruction of the tooth-supporting tissues and - if left untreated - eventually tooth loss. Periodontitis affects up to 50% of the population in the United States of America, and is classified in an aggressive and a chronic form depending on genetic factors, age of onset, speed and severity of attachment loss.

The onset of periodontitis is caused by an immunologic imbalance between host immune cells and residing microorganisms in subgingival pockets. The host immune cells are capable of enhancing both a protective and a destructive inflammatory response towards the microorganisms through the release of inflammatory mediators e.i. proinflammatory and antiinflammatory cytokines.

The role of antibodies in periodontitis is also unclear. Some studies show an excessive antibody level against bacteria associated with periodontitis e.g. Porphyromonas gingivalis (P.g.).

In general, this study contributes to a profound understanding of the host immune cells role in the onset and pathogenesis of periodontitis by comparing healthy versus diseased donors immunologic responses toward pathogene and apathogene microorganisms and their genetic background.

Enrollment: 90 patients

Sex: All

Ages: 19 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion criteria for chronic periodontitis donors:

• 50-60 years of age.
• Interproximal attachment loss at minimum 3 teeth besides molars and incisors.
• Clinical attachment loss at minimum 10 sites identified by bleeding and pus upon probing.
• Visible radiographic bone loss.
• Medically healthy donors.

Inclusion criteria for aggressive periodontitis donors:

• 19-40 years of age.
• Interproximal attachment loss at minimum 3 teeth besides molars and incisors.
• Clinical attachment loss at minimum 10 sites identified by bleeding and pus upon probing.
• Visible radiographic bone loss.
• Medically healthy donors.

Inclusion criteria for healthy donors: (age: 19-40 years; 50-60 years)

• No sign of inflammatory conditions or other general systemic diseases.
• Medically healthy donors.

Exclusion criteria for all groups:

• Pregnant and breastfeeding.
• Antibiotic treatment within 6 months.
• Suffer from periodontal manifestations caused by systemic diseases e.i. genetic diseases, haematologic anomalies or syndromes.

Trial design

90 participants in 3 patient groups

Chronic periodontitis donors

Description:

* Donors are medically healthy. * Slow to moderate attachment loss and bone destruction. * Good correlation between etiological factors and serverity of attachment loss.

Treatment:

Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria

Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers

Genetic: Analysis of selected single nucleotide polymorphisms (SNPs)

Diagnostic Test: periodontitis-associated bacteria presence

Aggressive periodontitis donors

Description:

* Donors are medically healthy. * Rapid attachment loss and bone destruction. * Familial aggregation. * No correlation between etiological factors and serverity of attachment loss.

Treatment:

Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria

Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers

Genetic: Analysis of selected single nucleotide polymorphisms (SNPs)

Diagnostic Test: periodontitis-associated bacteria presence

Control donors

Description:

* Donors are medically healthy. * No sign of inflammatory conditions.

Treatment:

Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria

Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers

Genetic: Analysis of selected single nucleotide polymorphisms (SNPs)

Diagnostic Test: periodontitis-associated bacteria presence