Interaction of Salivary Proteins and Polyphenols

Purdue University

Status: Completed

Conditions: Diet Habit; Taste, Altered; Diet Modification; Saliva Altered

Treatments: Other: Chocolate milk substitute with gelatin; Other: Chocolate milk substitute; Other: Chocolate Milk

Study type: Interventional

Funder types: Other

Identifiers

NCT03501238

082-002

Details and patient eligibility

About

Participants will be chosen through an initial sensory test to find high and low-perceivers of astringency. Selected participants will drink a chocolate milk or milk substitute beverage for one week each with washout weeks in between. At the end of each week participants will taste and rate multiple beverages for their astringency and other sensory properties. Participants will provide saliva samples weekly for proteomic analysis and (potentially) analysis of polyphenols. Participants will also be asked to provide a 24 hour dietary recall once per week. Data will be analyzed to determine if there is a relationship between polyphenol exposure, astringency ratings, and salivary protein composition.

Enrollment: 63 patients

Sex: All

Ages: 18 to 45 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• High or low perceivers of astringency (from initial screening)

Exclusion criteria

• Current smokers (within 30 days), dairy/nut/cocoa allergies, piercings in mouth, mid-range perceiver of astringency, issues with taste, smell, swallowing or choking.

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Single Blind

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63 participants in 6 patient groups

BSG

Experimental group

Description:

Participant receives bovine milk, then milk substitute, then milk substitute treated with gelatin

Treatment:

Other: Chocolate Milk

Other: Chocolate milk substitute

Other: Chocolate milk substitute with gelatin

BGS

Experimental group

Description:

Participant receives bovine milk, then milk substitute with gelatin, then milk substitute

Treatment:

Other: Chocolate Milk

Other: Chocolate milk substitute

Other: Chocolate milk substitute with gelatin

SBG

Experimental group

Description:

Participant receives milk substitute, then bovine milk, then milk substitute with gelatin.

Treatment:

Other: Chocolate Milk

Other: Chocolate milk substitute

Other: Chocolate milk substitute with gelatin

SGB

Experimental group

Description:

Participant receives milk substitute, then milk substitute with gelatin, then bovine milk

Treatment:

Other: Chocolate Milk

Other: Chocolate milk substitute

Other: Chocolate milk substitute with gelatin

GSB

Experimental group

Description:

Participant receives milk substitute with gelatin, then milk substitute, then bovine milk.

Treatment:

Other: Chocolate Milk

Other: Chocolate milk substitute

Other: Chocolate milk substitute with gelatin

GBS

Experimental group

Description:

Participant receives milk substitute with gelatin, then bovine milk, then milk substitute.

Treatment:

Other: Chocolate Milk

Other: Chocolate milk substitute

Other: Chocolate milk substitute with gelatin