Interest of Rapid Typing in Adenovirus Infections. (TYPADENO)

University Hospital of Bordeaux

Status: Completed

Conditions: Genotyping; Adenovirus Disease; Adenovirus

Study type: Observational

Funder types: Other

Identifiers

NCT04056546

CHU BX 2011/08

Details and patient eligibility

About

Proposal of a "rapid typing" technique by a new real-time PCR method, simpler, faster and cheaper than nucleotide sequencing (reference method) for rapid typing in Adenovirus infections.

Full description

Adenoviruses cause many infections, mainly respiratory and gastroenteric, in pediatrics.

They also behave as opportunistic agents in 10% of hematopoietic stem cell recipients. In these immunocompromised persons, these infections are all the more frequent as the graft is of placental origin and the recipient a child (20%). They are then willingly disseminated and potentially deadly; their treatment is based on cidofovir, a viral agent that is very sensitive to handling.

Adenoviruses, which have a high genetic diversity, are classified into 7 types. According to some observations, still limited, some types of Adenovirus would show a higher pathogenicity (types C and A). Thus, viral typing may be prognostic for immunocompromised patients, justifying the initiation of an earlier specific treatment when identifying certain more pathogenic viral types, in addition to measuring the viral load, already performed by quantitative PCR.

For this purpose, this study proposes a "fast typing" technique by new real-time PCR method, simpler, faster and cheaper than nucleotide sequencing, reference method.

Enrollment: 296 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• viral load greater than or equal to 10 ^ 4 copies / mL (blood and body fluids) or copies / gram of stool or copies / μg of DNA (biopsies).

Exclusion criteria

• Samples identified as positive in Adenovirus, not re-analyzable (insufficient sample quantity, alteration during thawing).