Interleukin-1β (rs 1143627 T/C) Gene Polymorphism May Increase the Risk of Developing LDD in Turkish Population

Yeditepe University

Status: Completed

Conditions: Inflammation; Low Back Pain; Lumbar Disc Degeneration

Study type: Observational

Funder types: Other

Identifiers

NCT05601739

YeditepeU-DilekÜ-001

Details and patient eligibility

About

This study aims to show the association between the Interleukin-1β (rs 1143627 T/C) gene polymorphism and the patient's pain level, radiological features, functional disability, and spinal flexibility.

Full description

Background: Lumbar disc degeneration (LDD), a complex process characterized by phenotypic and genotypic changes, causes low back pain.

Aims: This study aims to show the association between the Interleukin-1β (rs 1143627 T/C) gene polymorphism and the patient's pain level, radiological features, functional disability, and spinal flexibility.

Method: A hospital-based case-control study included 50 LDD patients and 44 healthy controls. In this study, Taqman allelic discrimination analysis was performed for the genotype of the Interleukin-1β (rs 1143627 T/C) gene polymorphism. Differences in Interleukin-1β allele frequencies and genotypes were evaluated between patients and controls. Relationships between allele presence/genotype and disease risk are reported as odds ratios (ORs) with 95% confidence intervals (CIs). The Pfirrmann classification was used to grade the level of disc degeneration on MRI scans, and the McNab's classification was used to classify disc displacements. The pain levels of the patient group were evaluated with VAS and their functional disability with ODI. Spinal flexibility was evaluated using the Modified Schober method and a digital inclinometer.

Enrollment: 94 patients

Sex: All

Ages: 18 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients with chronic low back pain (over 3 months
• Aged 18-60 years old
• With magnetic resonance imaging (MRI) evidencing LDD with Pfirrmann classification

Exclusion criteria

• Cauda equina syndrome
• Lumbar spinal stenosis
• Spinal deformities (scoliosis, kyphosis, spondylolisthesis)
• Vertebral fractures
• Inflammatory rheumatic diseases
• Diabetic neuropathy
• Pregnancy
• Drug and alcohol dependence
• Psychiatric illness
• Oncologic diseases
• Who refuse to sign the consent form and donate a blood sample for analysis of genomic DNA.

Trial design

94 participants in 2 patient groups

Lumbar degenerative disc disease

Description:

The case group was made of 50 patients, 24 women (48%) and 26 men (52%) with several clinical symptoms suggestive of LDD and the condition confirmed by MRI.

Healthy

Description:

The control group was made of 44 healthy volunteers.