Interleukin-2 (IL-2), Interleukin-5 (IL-5) and Interleukin-17 (IL-17) Levels of Patients With Stainless Steel Crowns

Yuzuncu Yıl University

Status: Completed

Conditions: Nickel Sensitivity; Saliva; Dental Crown

Treatments: Genetic: Salivary Collection; Diagnostic Test: Ph and ion level measurement of saliva

Study type: Observational

Funder types: Other

Identifiers

NCT06439095

YYU-BAVCI-01

Details and patient eligibility

About

The aim of this study is to investigate whether nickel hypersensitivity following stainless steel crown treatment in children aged 3-9 leads to an increase in IL-2, IL-5, and IL-17 levels by analyzing saliva samples. By doing so, the study seeks to prevent potential hypersensitivity reactions after stainless steel crown treatment.

The main question it aims to answer is:

Which interleukin level increases in the controls 1 week after stainless steel crowns are applied? Researchers will investigate whether there is a significant increase in interleukin levels in children's saliva before and after the procedure.

Participants will:

They will provide saliva samples before stainless steel crowns are applied They will visit the clinic after 1 week for check-ups and tests.

Full description

Expression levels of IL-2, IL-5 and IL-17 in the saliva of individuals aged 3-9 years who underwent stainless steel crown will be recorded before and after the procedure. Before the saliva sample collection procedure, the patient will be asked to rinse their mouth with sterile water and then spit into sterile plastic cups. After collecting approximately 5 ml of saliva, the saliva will be transferred to appropriate eppendorfs and stored at -40 degrees Celsius. For the second post-procedure, the same algorithms will be applied for saliva collection seven days after the crown procedure. Also ph value and ion levels of saliva will be measured.

Enrollment: 12 patients

Sex: All

Ages: 3 to 9 years old

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

**Inclusion Criteria:**

• Patients with teeth requiring stainless steel crowns due to having caries on two or more surfaces.
• Pediatric patients between 3-9 years old

**Exclusion Criteria:**

• Patients having any dental materials, such as fillings, fissure sealants, root canal treatments, or space maintainers
• Patients with known allergies to various foods and materials.
• Patients with systemic diseases that affect the immune system.
• Patients with a history of chemotherapy or radiotherapy.

Trial design

12 participants in 1 patient group

Stainless Steel Crown Group

Description:

Participants in this group received stainless steel crowns on posterior teeth. Salivary samples were collected before treatment (pre-treatment) and 7 days after treatment. IL-2, IL-5, and IL-17 gene expression levels were measured using Real-Time PCR. Additionally, salivary pH and the concentration of nickel, chrome ions were analyzed using atomic absorption spectrophotometry.

Treatment:

Diagnostic Test: Ph and ion level measurement of saliva

Genetic: Salivary Collection