Intrauterine Human Chorionic Gonadotrophins (hCG) and Endometrial Treg Cells

The Egyptian IVF-ET Center

Status: Completed

Conditions: Infertility

Treatments: Drug: human chorionic gonadotrophin

Study type: Interventional

Funder types: Other

Identifiers

NCT01064219

hCG and Treg cells

Details and patient eligibility

About

Intrauterine injection of hCG around the time of implantation may increase endometrial Treg cells and improve implantation. It has been demonstrated that regulatory T cells expand during pregnancy. hCG was found to be secreted by the embryo immediately after fertilization and has chemoattractant properties to Treg cells.

Full description

Aim of the work:

To study the effect of intrauterine injection of human chorionic gonadotrophin on the endometrial T regulatory cells (Treg cells)and the electromicroscopic endometrial characteristics.

Background:

It has been demonstrated that regulatory T cells expand during pregnancy and are present at the feto-maternal interface at very early stages in pregnancy.

Rational:

Intrauterine injection of hCG around the time of implantation may increase endometrial Treg cells and improve implantation.

Methods:

Intrauterine injection of 100 IU hCG will be done followed by endometrial biopsy to check for Treg cells and pinopodes.

Enrollment: 20 patients

Sex: Female

Ages: 20 to 38 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Male factor infertility
• Ovulatory factor infertility
• Unexplained infertility
• Female age less than 39

Exclusion criteria

• Azoospermia
• Tubal factor infertility
• Pelvic endometriosis

Trial design

Primary purpose: Diagnostic

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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20 participants in 1 patient group

intrauterine hCG

Experimental group

Description:

intrauterine injection of 100 hCG followed by endometrial biopsy

Treatment:

Drug: human chorionic gonadotrophin