Investigating the Bactericidal Effect of Probiotics on Chromogenic Bacteria in Children An In Vivo and In Vitro Study

Ain Shams University

Status and phase: Completed; Phase 4

Conditions: Black Stains

Treatments: Drug: Probiotic with Prebiotic

Study type: Interventional

Funder types: Other

Identifiers

NCT06834815

FDASU-Rec IR102420

Details and patient eligibility

About

To investigate the bactericidal effect of probiotics (Streptococcus salivarius M18 and Lactobacillus reuteri) on two types of bacteria (Aggregatibacter actinomycetemcomitans and Actinomyces naeslundii) causing black stains of teeth in vitro.

Full description

In vitro:

An unstimulated salivary sample will be taken from patients having black stains. The two types of bacteria of each condition will be cultured, amplified, and measured in the laboratory from the saliva of these patients. After that, one type of probiotics will be applied to these bacteria separately and the same bacterial measurement will be done to evaluate the bactericidal effect of the probiotics on these bacteria, aiming to find the minimal inhibitory concentration of the probiotics.

Enrollment: 9 patients

Sex: All

Ages: 6 to 14 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients with black stains on teeth scored via modified Lobene index scores 1-3.
• Medically free patients

Exclusion criteria

• Stains other than black.
• Patients on antibiotic treatment or any other medication for the last 2 weeks.
• Patients allergic to prophylaxis powder.
• Parents refusing to participate

Trial design

Primary purpose: Treatment

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

---

9 participants in 1 patient group

before and after probiotic administration

Experimental group

Description:

all patients will submit an unstimulated salivary sample of 1ml will in sealed test tube * then the patients will receive probiotics for 14 days . * after 14 days and another salivary sample will be collected

Treatment:

Drug: Probiotic with Prebiotic