Investigation of Supragingival Plaque Microbiome in Early Childhood Caries

Trakya University

Status: Completed

Conditions: Early Childhood Caries

Treatments: Genetic: Collection of supragingival plaque from caries free children

Study type: Observational

Funder types: Other

Identifiers

NCT04456335

2018/269

Details and patient eligibility

About

The purpose of this study was to characterize the supragingival plaque microbiome in children with early childhood caries and identify potential microorganisms associated with caries.

Full description

Sixty children with and without early childhood caries were recruited from Trakya University Faculty of Dentistry Department of Pediatric Dentistry. Oral examinations were performed by the same examiner according to diagnostic criteria of World Health Organisation (WHO) and The International Caries Detection and Assessment System (ICDAS) . Mothers' completed a structured questionnaire on the socio-demographic informations, child's feeding practices and oral hygiene habits. After the oral examination, supragingival plaque samples were collected from the posterior tooth surfaces. Supragingival plaque samples were examined with the Illumina MiSeq new generation sequencing method.

Enrollment: 60 patients

Sex: All

Ages: 36 to 71 months old

Volunteers: No Healthy Volunteers

Inclusion criteria

• 36-71 months old children
• No history of any medical problem
• No history of antibiotic in the three months prior to sampling
• Any sign of early childhood caries,
• Caries free children
• Mothers accepted to complete a structured questionnaire
• Children who cooperate for dental examination and sample collection

Exclusion criteria

• Children older than 71 months
• Medically compromised
• Children with antibiotic use history at last three months
• Children refused dental examination and plaque collection
• Mothers' refused to complete questionnare

Trial design

60 participants in 2 patient groups

Caries-free

Description:

Caries-free children

Treatment:

Genetic: Collection of supragingival plaque from caries free children

Early childhood caries

Description:

Children with early childhood caries

Treatment:

Genetic: Collection of supragingival plaque from caries free children