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Investigation of the Effects of a Bifidobacterium Breve Strain on Fat Loss in Healthy Adults

M

Morinaga Milk Industry

Status

Completed

Conditions

Overweight and Obesity
Obesity

Treatments

Dietary Supplement: Placebo
Dietary Supplement: B. breve strain

Study type

Interventional

Funder types

Industry

Identifiers

Details and patient eligibility

About

Overweight has become a critical issue in North America and the market value of weight loss products is expected to rise as the population becomes more health-conscious and aware of the risks associated with excess body weight. This randomized, placebo-controlled, clinical trial investigates the effect of Bifidobacterium breve supplementation with exercise intervention on fat loss.

Full description

In early adulthood, excess body weight is a risk factor associated with several health complications later on in life and probiotics have been used for decades for maintaining intestinal health, and in recent years probiotics have been proposed for weight management. This randomized, placebo-controlled, clinical trial investigates the effect of Bifidobacterium breve supplementation with exercise intervention on fat loss.

Enrollment

95 patients

Sex

All

Ages

20 to 65 years old

Volunteers

Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion Criteria:

  • Male or female between 20 and 65 years of age, inclusive

  • BMI from 25.0 to 29.9 kg/m2, inclusive

  • Female participants are not of child-bearing potential, defined as females who have undergone a sterilization procedure (e.g. hysterectomy, bilateral oophorectomy, bilateral tubal ligation, complete endometrial ablation) or have been post-menopausal (natural or surgically) for at least 1 year prior to screening

  • Or, Females of child-bearing potential must have a negative baseline urine pregnancy test and agree to use a medically approved method of birth control for the duration of the study. All hormonal birth control must have been in use for a minimum of three months. Acceptable methods of birth control include:

    1. Hormonal contraceptives including oral contraceptives, hormone birth control patch (Ortho Evra), vaginal contraceptive ring (NuvaRing), injectable contraceptives (Depo-Provera, Lunelle), or hormone implant (Norplant System)
    2. Double-barrier method
    3. Intrauterine devices
    4. Non-heterosexual lifestyle or agrees to use contraception if planning on changing to heterosexual partner(s)
    5. Vasectomy of partner at least 6 months prior to screening
  • Self-reported stable body weight for the past 3 months defined as not having gained or lost more than 5 kg of body weight throughout the 3 months prior to baseline

  • Participants with the following body fat percentages as determined by Bioelectrical Impedance Analysis (BIA):

    1. Female: ≥ 30%
    2. Male: ≥ 20%
  • Agrees to follow the diet and exercise guidelines for the duration of the study

  • Willingness to complete questionnaires, records, and diaries associated with the study, to complete all clinic visits, and provide stool samples

  • Provide voluntary, written, informed consent to participate in the study

  • Healthy as determined by medical history, laboratory results and physical exam as assessed by the Qualified Investigator (QI)

Exclusion Criteria

  • Women who are pregnant, breastfeeding or planning to become pregnant during the trial

  • Allergy, sensitivity, or intolerance to the investigational product's active or inactive ingredients

  • Clinically significant abnormal laboratory results at screening as assessed by the QI

  • Current or history of any significant gastrointestinal disease requiring medication (e.g. GERD, gastroenteritis)

  • Irregular sleep schedule

  • Chronic diarrhea or constipation

  • Participants with hypertension and are on antihypertensive medication

  • Type I or Type II diabetes

  • Participants with hyperlipidemia and are on medication

  • Self-reported sleep apnea

  • Self-reported current or pre-existing thyroid condition. Treatment on a stable dose of medication for at least 3 months will be considered by the QI

  • Unstable metabolic disease or chronic diseases as assessed by the QI

  • History of or current diagnosis with kidney and/or liver diseases as assessed by the QI on a case-by-case basis, with the exception of history of kidney stones in participants who are symptom-free for 6 months

  • Significant cardiovascular event in the past 6 months. Participants with no significant cardiovascular event on stable medication may be included after assessment by the QI on a case by case basis

  • Major surgery in the past 3 months or individuals who have planned surgery during the trial period. Participants with minor surgery will be considered on a case-by-case basis by the QI

  • Cancer, except skin cancers completely excised with no chemotherapy or radiation with a follow up that is negative. Volunteers with cancer in full remission for more than five years after diagnosis are acceptable

  • Individuals with an autoimmune disease or are immune-compromised

  • Self-reported HIV-, Hepatitis B- and/or C-positive diagnosis

  • Blood/bleeding disorders as determined by laboratory results

  • Self-reported mental or neuropsychological condition and/or cognitive impairment that, in the QI's opinion, could interfere with study participation

  • Metal implants that may affect the DXA scan results will be assessed on a case- by-case basis by the QI.

  • Current use of prescribed medications listed in Section Prescribed Medications as follows:

    1. Beta-blockers and thiazide diuretics (within 4 weeks of baseline)
    2. Weight loss medication (within 4 weeks of baseline)
    3. Lipid-lowering medications (within 4 weeks of baseline)
    4. Anticoagulants and coagulants (within 4 weeks of baseline)
    5. Sleep medication
    6. Selective serotonin reuptake inhibitors (SSRI)
    7. Antibiotics
    8. Non-steroidal anti-inflammatory drugs (NSAIDs)
    9. Proton pump inhibitors (PPIs)
    10. Metformin (unless on a stable dose for the last 6 months)
  • Current use of over-the-counter medications, supplements, foods and/or drinks as follows:

    1. OTC NSAIDs (PRN use is acceptable)
    2. OTC blood pressure medication or supplements (within 4 weeks of
    3. baseline)
    4. Lipid metabolising supplements (within 4 weeks of baseline)
    5. Fish oil and omega-3 supplements
    6. Red yeast rice
    7. Plant sterols and stanols
    8. OTC medication or supplements marketed for weight loss (within 4 weeks
    9. of baseline)
    10. Vitamin E supplements (within 4 weeks of baseline)
    11. Coagulant/anticoagulant supplements (within 4 weeks of baseline)
    12. PPIs
  • Use of cannabinoid products within 60 days of baseline. History of cannabis used will be assessed on a case by case basis by the QI

  • Use of tobacco products within 60 days of baseline

  • Self-reported alcohol or drug abuse within the last 12 months

  • High alcohol intake (average of > 2 standard drinks per day or > 10 per week)

  • Current employment that calls for shift work or have worked shift work in the last 3 weeks

  • Participation in other clinical research trials 30 days prior to screening

  • Blood donation 30 days prior to screening, during the study, or a planned donation within 30-days of the last study visit

  • Individuals who are unable to give informed consent

  • Any other condition, that, in the opinion of the QI, may adversely affect the participant's ability to complete the study or its measures, or which may pose a significant risk to the participant

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

Quadruple Blind

95 participants in 2 patient groups, including a placebo group

Placebo
Placebo Comparator group
Description:
Placebo delivered in capsule format. Participants will be instructed to take 2 capsules of placebo for 4 weeks during the run-in period. On day 1 participants will be instructed to take 2 capsules of placebo in the morning before breakfast for 12 weeks.
Treatment:
Dietary Supplement: Placebo
B. breve
Experimental group
Description:
Capsule containing B breve strain. Participants will be instructed to take 2 capsules of placebo for 4 weeks during the run-in period. On day 1 participants will be instructed to take 2 capsules of B. breve strain in the morning before breakfast for 12 weeks.
Treatment:
Dietary Supplement: B. breve strain

Trial documents
2

Trial contacts and locations

1

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Central trial contact

Andrew Charrette, MSc; Malkanthi Evans, PhD

Data sourced from clinicaltrials.gov

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