Isolation of Vitamin D Binding Protein Isoforms From Human Volunteers

Universitaire Ziekenhuizen KU Leuven

Status: Active, not recruiting

Conditions: Vitamin D Deficiency

Treatments: Other: Blood draw

Study type: Observational

Funder types: Other

Identifiers

NCT02258035

s57027

Details and patient eligibility

About

The purpose of this study is to isolate genotype-specific DBP/Gc from homozygous human volunteers and use these purified proteins to cross-validate existing or novel assays for this serum protein and to evaluate the binding characteristics of these variants of DBP/Gc for different vitamin D metabolites.

Enrollment: 24 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Generally healthy adults (≥ 18 years), non pregnant, with known DBP/Gc genotype

Exclusion criteria

• Inability to provide written informed consent
• No children or pregnant women
• Persons with known abnormal serum proteins

Trial design

24 participants in 3 patient groups

DBP/Gc1f-1f genotype

Description:

Human volunteers homozygous for the 1f-1f (fast) genotype of DBP.

Treatment:

Other: Blood draw

DBP/Gc1s-1s genotype

Description:

Human volunteers homozygous for the 1s-1s (slow) genotype of DBP.

Treatment:

Other: Blood draw

DBP/Gc 2-2 genotype

Description:

Human volunteers homozygous for the 2-2 genotype of DBP.

Treatment:

Other: Blood draw