Laser Assisted Sperm Selection of Viable Immotile Testicular Sperm in Azoospermic Infertile Men

Sohag University

Status: Completed

Conditions: IVF; Azoospermia

Treatments: Procedure: Hypo-osmotic Swelling Test; Device: Laser Assisted Immotile Sperm Selection

Study type: Interventional

Funder types: Other

Identifiers

NCT04675164

LAISS

Details and patient eligibility

About

This study aims to investigate the outcome of ICSI after using LAISS for selection of viable immotile testicular sperm in azoospermic infertile men.

Patients will be randomly assigned to 2 groups. In (LAISS) group, viable immotile testicular sperms will be selected before ICSI using laser assisted immotile sperm selection (study group). In (HOST) group, viable immotile testicular sperms will be selected before ICSI using hypo-osmotic swelling test (control group).

Enrollment: 168 patients

Sex: All

Ages: 18 to 35 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Eligible couples are those undergoing ICSI due to azoospermia with testicular biopsy positive for sperm (either fresh or cryopreserved) following testicular sperm extraction (TESE) or aspiration (TESA).

Exclusion criteria

• Cases with female factor infertility or woman age above 35 years will be excluded.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Triple Blind

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168 participants in 2 patient groups

LAISS group

Active Comparator group

Description:

Viable immotile testicular sperms will be selected before ICSI using laser assisted immotile sperm selection (study group).

Treatment:

Device: Laser Assisted Immotile Sperm Selection

HOST group

Active Comparator group

Description:

Viable immotile testicular sperms will be selected before ICSI using hypo-osmotic swelling test (control group).

Treatment:

Procedure: Hypo-osmotic Swelling Test