LC-MS/MS 25(OH)D Status in a Large Cohort

Azienda Ospedaliera di Bolzano

Status: Completed

Conditions: 25(OH)D Measurement and 25(OH)D Deficiency

Treatments: Diagnostic Test: 25(OH)D measurement, PTH measurement

Study type: Observational

Funder types: Other

Identifiers

NCT03388203

0055719-BZ

Details and patient eligibility

About

The present study assessed serum 25(OH)D in a cohort of 74235 outpatients by liquid-chromatography-tandem-mass-spectrometry (LC-MS/MS).

The investigators studied the distribution of serum 25(OH)D concentrations in males and females of different age groups, the prevalence of measurable amounts of 25(OH)D2 and seasonal variability.

Full description

Measurements were performed under routine circumstances. Samples were collected in serum tubes with clot-activator. As per routine procedure, samples were centrifuged upon arrival in the lab and stored at 4° C until measurement. 25(OH)D3 and 25(OH)D2 were quantitated separately. Results from subjects <18 years were excluded from the analysis. The study was approved by the local Ethics Committee.

Enrollment: 74,235 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• >18 years, outpatients;

Exclusion criteria

• <18 years, inpatients;