LED Photobiomodulation Improves Radial Artery Vascular Function in Vitro

University of Sao Paulo

Status: Completed

Conditions: Vascular Dysfunction

Treatments: Device: Light-emitting diode photobiomodulation therapy

Study type: Interventional

Funder types: Other

Identifiers

NCT07041697

4.829.712

Details and patient eligibility

About

LED photobiomodulation therapy (PBMT) is widely recognized for its beneficial effects in several clinical conditions; however, its potential in modulating vascular function is not yet fully established. Objective: This study aimed to evaluate the application of PBMT by LED (light-emittion diode) using a combined red and near-infrared light spectrum, with a focus on improving the vascular function of the radial artery in vitro. Methodology: A total of 30 radial artery segments were studied and divided into three groups of 10 segments: the PBMT LED group combined with red and infrared light (R + IR), the red light (R) group, and the infrared light (IR) group. Prior to light irradiation at wavelengths of 650 nm and 940 nm, vascular function in these segments was pharmacologically inhibited using L-NAME. The drugs phenylephrine, acetylcholine, and sodium nitroprusside were used to determine if irradiation could restore the inhibited vascular functions. Superoxide anion levels, an important reactive oxygen specie (ROS), were measured using a chemiluminescence assay with lucigenin. The bioavailability of nitric oxide was evaluated using a Griess Reagent Kit assay, while nitric oxide production was assessed through the DAF fluorescence assay. The vascular inflammation was determined by the Intercellular Adhesion Molecule 1 (ICAM-1) expression.

Enrollment: 30 patients

Sex: All

Ages: 20 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients undergoing coronary artery bypass grafting using the radial artery

Exclusion criteria

• Patients with poorly compliant radial arteries or those who underwent coronary artery bypass grafting without use of the radial artery were excluded from the study

Trial design

Primary purpose: Prevention

Allocation: Non-Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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30 participants in 3 patient groups

Photobiomodulation Using Red LED Light at 650 nm Wavelength

Experimental group

Description:

The three arms of the study aim to evaluate the effectiveness of different LED light spectra on vasodilation.

Treatment:

Device: Light-emitting diode photobiomodulation therapy

Photobiomodulation Using Red LED Light at 940 nm Wavelength

Experimental group

Description:

The three arms of the study aim to evaluate the effectiveness of different LED light spectra on vasodilation.

Treatment:

Device: Light-emitting diode photobiomodulation therapy

Photobiomodulation with Combined Red (650 nm) and Infrared (940 nm) LED Light

Experimental group

Description:

The three arms of the study aim to evaluate the effectiveness of different LED light spectra on vasodilation.

Treatment:

Device: Light-emitting diode photobiomodulation therapy