LVA on Serum Profiling in Patients With Lymphedema

Chang Gung Medical Foundation

Status: Completed

Conditions: Lymphedema

Treatments: Other: post-LVA; Other: pre-LVA

Study type: Observational

Funder types: Other

Identifiers

NCT04552938

201800306B0

Details and patient eligibility

About

The correlation between oxidative stress and increased lymphedematous limb volume lacks supporting evidence. Lymphedema patients are prone to cellulitis. This study aims to investigate the link between oxidative stress, limb volume, and cellulitis after supermicrosurgical lymphaticovenous anastomosis (LVA).

Full description

In this study, we focused on the changes in serum antioxidant biomarkers before and after LVA, detected by isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis, enzyme-linked immunosorbent assay (ELISA), and Oxidative Stress Panel Kit. For clinical correlation, magnetic resonance (MR) volumetry was implemented for precise lymphedematous limb volume measurements. Correlations were made between changes in oxidative stress, limb volume, and cellulitis before and after LVA.

Enrollment: 26 patients

Sex: All

Ages: 20 to 100 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• patients with lower limb lymphedema confirmed with lymphoscintigraphy
• unilateral lower limb lymphedema
• lymphedema duration > 2 years
• no active infection
• no prophylactic antibiotic use for at least one month before LVA
• no tumor recurrence or metastasis
• no consumption of antioxidants, such as vitamin E or ascorbic acid

Exclusion criteria

• Patients with upper limb and bilateral lower limb lymphedema

Trial design

26 participants in 1 patient group

Patients underwent supermicrosurgical LVA

Description:

Patients underwent supermicrosurgical LVA between June 2018 and May 2019.

Treatment:

Other: pre-LVA

Other: post-LVA