Macrophage Markers in Periodontal and Peri-implant Health and Disease

Biruni University

Status: Completed

Conditions: Periodontitis; Peri-Implantitis

Study type: Observational

Funder types: Other

Identifiers

NCT05242354

Peri-implant macrophage

Details and patient eligibility

About

Macrophage surface markers (CD80, CD163 and CD206) will be evaluated in periodontally healthy gingiva, healthy peri-implant mucosa, and periodontitis and peri-implantitis lesions.

Full description

Soft tissue biopsies obtained from clinically healthy gingiva, healthy peri-implant mucosa, Stage III Grade C periodontitis lesions and peri-implantitis lesions will be evaluated.

Healthy peri-implant samples are obtained from submerged implants during exposure surgery. Healthy gingiva samples are obtained from individuals receiving crown lengthening, gingivectomy or tooth extraction as excess tissue during the corresponding treatment. Samples from peri-implantitis and periodontitis (pocket depth ≥6 mm, bleeding on probing+) are obtained during initial periodontal treatment / debridement procedure with a single stroke at the pocket wall.

The samples will be grinded and ultrasonicated. Western blotting will be used for determination of CD80, CD163 and CD206 in biopsy specimens. ImageJ will be used for intensity analysis of the bands.

Enrollment: 36 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• diagnosed with generalized Grade C Stage III periodontitis
• diagnosed with peri-implantitis
• periodontally healthy individuals with dental implants in the primary healing period
• periodontally healthy individuals who are in need of crown lengthening / gingivectomy or extraction

Exclusion criteria

• systemic disease
• smoking / former smokers
• regular medicine intake
• antibiotic or anti-inflammatory medicine intake in the last 3 months
• pregnancy or lactation

Trial design

36 participants in 4 patient groups

Periodontal health

Description:

Periodontally healthy individuals (2017 Classification of Periodontal Diseases and Conditions) who are planned to receive crown lengthening / gingivectomy of tooth extraction involving at least one site with pocket depth ≤3mm, bleeding on probing -

Peri-implant health

Description:

Periodontally healthy individuals (2017 Classification of Periodontal Diseases and Conditions) with at least one partially-exposed dental implant with no bone loss prior to the prosthetic rehabilitation

Periodontitis

Description:

Generalized Stage III Grade C periodontitis patients (2017 Classification of Periodontal Diseases and Conditions) having at least one site with pocket depth ≥6mm, bleeding on probing +

Peri-implantitis

Description:

Individuals with at least one implant site with radiographic bone loss, pocket depth ≥6mm and bleeding on probing +