Macrophage/Monocyte Driven Inflammation and Anesthetic Sensitivity in Aging (MACRO-AGE)

University of Chile

Status

Not yet enrolling

Conditions

Inflammaging

Aging

Inflammation Biomarkers

Inflammation

Blood-Brain Barrier Permeability

Monocyte

Anesthesia Brain Monitor

Anesthesia Depth Monitoring

Study type

Observational

Funder types

Other

Identifiers

NCT07372417

OAIC N°1538/25

Details and patient eligibility

About

The goal of this observational study is to determine whether macrophage/monocyte mediated inflammatory signaling contributes to reduced anesthetic requirements in older adults undergoing major abdominal surgery. The main questions it aims to answer are:

Is there a difference in anesthetic dosing requirements (minimum effective dose) between young and older patients undergoing major abdominal surgery?
How do electroencephalographic (EEG) signatures under anesthesia correlate with age and systemic inflammatory markers?
Is there an association between age, levels of circulating inflammatory cytokines, and monocyte/macrophage phenotypes with anesthetic requirements?

If there is a comparison group: Researchers will compare older adult patients undergoing major abdominal surgery to younger adult patients undergoing major abdominal surgery to see if macrophage/monocyte-mediated inflammatory signaling influences anesthetic sensitivity and the risk of postoperative neurocognitive complications in the older population.

Participants will:

Receive general anesthesia for major abdominal surgery, with continuous recording of anesthetic dose requirements.
Undergo electroencephalographic (EEG) monitoring during the anesthetic period.
Provide blood samples for the measurement of circulating inflammatory cytokines and the assessment of monocyte phenotypes.
Provide peritoneal tissue samples (collected during surgery) to evaluate tissue macrophage populations.
Provide cerebrospinal fluid (CSF) samples to assess biomarkers of blood-brain barrier permeability.

Enrollment

60 estimated patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Age between 18-30 years or over 60 years old
Scheduled for abdominal surgery
General anesthesia planned with or without spinal anesthesia
Able and willing to provide written informed consent.

Exclusion criteria

Chemotherapy or systemic corticosteroid use within the past 2 weeks.
Pregnancy.
Active sepsis or systemic infection.
Emergency surgery.
Requirement of ketamine or dexmedetomidine during the first hour of anesthesia.
History or diagnosis of: stroke with persistent neurological deficit, dementia, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, central nervous system autoimmune disorders, systemic autoimmune diseases, or infectious diseases of the central nervous system.
Current treatment with immunomodulatory medications.
Psychiatric disorders with psychotic features.
Use of psychoactive or psychotropic recreational drugs within the week prior to surgery.