Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging (S48)

Purdue University

Status: Completed

Conditions: Flaccid Muscle Tone

Treatments: Procedure: muscle biopsy

Study type: Interventional

Funder types: Other

Identifiers

NCT02685319

1512016862

Details and patient eligibility

About

Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging

Full description

The aim of this study is to obtain human muscle samples for cell culturing to analyze muscle cell development in real-time to uncover factors that influence growth.

Enrollment: 7 patients

Sex: Male

Ages: 20 to 29 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

-Inclusion Criteria:

• men 20-29 yrs
• BMI between 20 and 34.9
• Fasting Glucose <110 mg/dL
• Blood Pressure <160/100mm Hg
• Non-Diabetic
• LDL-Cholesterol <160mg/dL
• Triacylglycerol <400 mg/dL
• Makers of kidney, liver, and heart functions within 10% of clinical normalcy
• <1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

Exclusion Criteria:

• men <20or>29 yrs

  • BMI between <20 and >34.9

  • Fasting Glucose >110 mg/dL

  • Blood Pressure >160/100mm Hg

  • Non-Diabetic

  • LDL-Cholesterol >160mg/dL

  • Triacylglycerol >400 mg/dL

  • Pre-existing kidney or liver conditions

    -->1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

  • Currently taking prescribed blood thinners

Trial design

Primary purpose: Other

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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7 participants in 1 patient group

muscle biopsy

Experimental group

Description:

a muscle sample will be taken from the mid-thigh (Vastus Lateralis) and analyzed

Treatment:

Procedure: muscle biopsy