Matrilysin Expression in Different Stages of Colorectal Tumors (MMP7)

University of Roma La Sapienza

Status: Completed

Conditions: Colorectal Cancer Stage III; Colorectal Cancer Stage IV; Colorectal Cancer Stage II; Colorectal Cancer Stage I; Colorectal Cancer Stage 0

Treatments: Procedure: colonic or colorectal resection

Study type: Observational

Funder types: Other

Identifiers

NCT01570452

andreapolistena1

Details and patient eligibility

About

Matrix metalloproteinases (MMPs) have been shown to be involved in cancer biology. Significant expression of MMP-7 (matrilysin) in colorectal cancer is mainly associated with metastatic disease even though it is expressed in most tumor states. Our purpose is to analyse MMP-7 in bowel and lymph nodes of different tumor stages and to evaluate its expression as a potential biomarker of cancer disease in patients surgically treated for benign and malignant colorectal tumors. Tumoral tissue, lymph nodes and serum samples from recruited Patients plus serum samples from healthy volunteers are analysed for matrilysin expression by histology, immunohistochemistry, ELISA and Western blotting.

If Matrilysin increases with increasing dysplasia and cancer disease stage in tumor tissue as well as in the regional lymph nodes it might be used as a complement in investigating suspected locally advanced cancer.

Enrollment: 38 patients

Sex: All

Ages: 40 to 83 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• benign colonic neoplasm not suitable for endoscopic treatment,
• malignant colonic neoplasm with indication to primary surgical treatment

Exclusion criteria

• neo-adjuvant radiotherapy,
• chemo-radiotherapy,
• language problems and withdrawal of consent

Trial design

38 participants in 4 patient groups

healthy volunteers

Description:

healthy subjects not affected by benign or malignant colonic disease

Treatment:

Procedure: colonic or colorectal resection

benign colonic tumor patients

Description:

patients affected by benign colonic tumor such as adenoma

Treatment:

Procedure: colonic or colorectal resection

cancer patient I-II stage

Description:

Patients affected by colonic cancer in I-II stage

Treatment:

Procedure: colonic or colorectal resection

cancer patients III-IV stage

Description:

Patients affected by colonic cancer in III-IV stage

Treatment:

Procedure: colonic or colorectal resection